Determination of nikethamide and N-ethylnicotinamide in the blood and urine of greyhounds.

Following the intramuscular administration of nikethamide to a series of greyhounds, both plasma and urine excretion levels were obtained. A qualitative urine screening procedure for both nikethamide and its major metabolite has been devised. The method involves solvent extraction, thin-layer and a two-system gas chromatographic system.