The effects of oestradiol on the acid-soluble nucleotides of rat uterus.
作者信息
Oliver J M, Kellie A E
出版信息
Biochem J. 1970 Sep;119(2):187-91. doi: 10.1042/bj1190187.
Abstract
- Techniques have been developed to measure the concentrations of the ribonucleotides of the immature rat uterus in vivo. Tissue was frozen rapidly in liquid nitrogen, ground to a fine powder, dispersed in frozen perchloric acid and thawed slowly. Nucleotides were separated from other acid-soluble constituents on short columns of polyethyleneimine-cellulose and the mixture was resolved into individual nucleotides by two-dimensional thin-layer chromatography on polyethyl-eneimine-cellulose plates. 2. The nucleotides of immature rat uterus consisted of approximately 75% of ATP-ADP, 10-12% each of GTP-GDP and UTP-UDP and less than 2% of CTP. 3. Injection of oestradiol (5mug) promoted a linear decrease in the amounts of purine nucleotides to approximately 60% of control values in 4-5h, followed by a return to greater than control values in 8-10h. Concentrations of the pyrimidine nucleotides remained constant for 4-6h and then increased to 200% of control at 12h after hormone treatment.
摘要
相似文献
1
The effects of oestradiol on the acid-soluble nucleotides of rat uterus.
Biochem J. 1970 Sep;119(2):187-91. doi: 10.1042/bj1190187.
2
Ion-exchange chromatography of nucleotides on polyethyleneimine cellulose columns: analysis of maize grain extracts.
Anal Biochem. 1968 Jan;22(1):35-46. doi: 10.1016/0003-2697(68)90256-x.
3
New method for the differentiation of ribonucleotides from deoxyribonucleotides.
Anal Biochem. 1969 May;29(2):210-22. doi: 10.1016/0003-2697(69)90304-2.
4
Direct evidence for the binding or compartmentalization of acid-soluble metabolites in oocyte cells.
J Cell Physiol. 1966 Oct;68(2):99-106. doi: 10.1002/jcp.1040680202.
5
Thin-layer electrophoresis of ribonucleotides.
Anal Biochem. 1969 Apr 4;28(1):313-7. doi: 10.1016/0003-2697(69)90185-7.
6
Free ribonucleotides of Physarum polycephalum.
Eur J Biochem. 1969 Apr;8(4):557-61. doi: 10.1111/j.1432-1033.1969.tb00563.x.
7
Analysis of ribonucleotides and deoxyribonucleotides using high-pressure liquid column chromatography.
J Chromatogr. 1970 Sep 16;51(2):203-9. doi: 10.1016/s0021-9673(01)96855-4.
8
[Separation of mono-, di-, and triphosphate nucleosides by thin layer chromatography on silica].
J Chromatogr. 1967 Dec;31(2):609-12. doi: 10.1016/s0021-9673(01)86131-8.
9
[Acid-soluble, UV-absorbing compounds of the guinea pig epidermis].
Arch Klin Exp Dermatol. 1967;228(2):179-87.
10
Effect of alkali on ribonucleoside-2',3'-monophosphates.
Acta Chem Scand. 1968;22(1):360. doi: 10.3891/acta.chem.scand.22-0360.
引用本文的文献
1
An in vivo study of the effects of ischaemia on uterine contraction, intracellular pH and metabolites in the rat.
J Physiol. 1994 Apr 15;476(2):349-54. doi: 10.1113/jphysiol.1994.sp020136.
2
The effects of oestradiol on nucleoside transport in rat uterus.
Biochem J. 1971 Jan;121(1):83-8. doi: 10.1042/bj1210083.
3
Progesterone and the metabolic control of the lactose biosynthetic pathway during lactogenesis in the rat.
Biochem J. 1973 Dec;136(4):1105-16. doi: 10.1042/bj1361105.
4
A reappraisal of the effects of adenosine 3':5'-cyclic monophosphate on the function and morphology of the rat prostate gland.
Biochem J. 1973 May;134(1):129-42. doi: 10.1042/bj1340129.
5
A possible role for 5-phosphoribosyl 1-pyrophosphate in the stimulation of uterine purine nucleotide synthesis in response to oestradiol-17 .
Biochem J. 1972 Jul;128(4):771-7. doi: 10.1042/bj1280771.
6
The effects of pregnancy and parturition on phosphorus metabolites in rat uterus studied by 31P nuclear magnetic resonance.
J Physiol. 1985 Nov;368:19-31. doi: 10.1113/jphysiol.1985.sp015844.
本文引用的文献
1
POSSIBLE SYNTHESIS OF POLYRIBONUCLEOTIDES OF KNOWN BASE-TRIPLET SEQUENCES.
Nature. 1965 Apr 3;206:93. doi: 10.1038/206093b0.
2
THE EFFECT OF OESTROGEN ON THE ACID-SOLUBLE NUCLEOTIDE CONTENT OF THE RABBIT VAGINA.
Acta Obstet Gynecol Scand. 1964;43:369-80. doi: 10.3109/00016346409162687.
3
RESOLUTION OF COMPLEX NUCLEOTIDE MIXTURES BY TWO-DIMENSIONAL ANION-EXCHANGE THIN-LAYER CHROMATOGRAPHY.
J Chromatogr. 1964 Oct;16:126-9. doi: 10.1016/s0021-9673(01)82446-8.
4
Thymidine diphosphate mannose and thymidine diphosphate rhamnose in Streptomyces grieus.
Biochem J. 1961 Oct;81(1):114-24. doi: 10.1042/bj0810114.
5
A simple procedure for removing salts from samples applied to ion-exchange thin-layer plates.
Anal Biochem. 1966 Jul;16(1):149-52. doi: 10.1016/0003-2697(66)90090-x.
6
Nucleotide pools and [6-14C]orotic acid incorporation in early regenerating rat liver.
Biochim Biophys Acta. 1966 Dec 21;129(3):445-59. doi: 10.1016/0005-2787(66)90060-8.
7
Effect of estrogen on uterine ATP levels.
Proc Soc Exp Biol Med. 1965 Oct;120(1):9-10. doi: 10.3181/00379727-120-30428.
8
Desalting of nucleic acid hydrolysates, nucleosides and bases by chromatography on poly-N-vinyl pyrrolidone.
J Chromatogr. 1969 Mar 25;40(2):299-302. doi: 10.1016/s0021-9673(01)96663-4.
9
Ion-exchange chromatography of tissue nucleotides.
J Chromatogr. 1969 Oct 28;44(2):331-41. doi: 10.1016/s0021-9673(01)92544-0.
10
Control by estrogen of genetic transcription and translation. Binding to chromatin and stimulation of nucleolar RNA synthesis are primary events in the early estrogen action.
Science. 1968 Aug 16;161(3842):649-61. doi: 10.1126/science.161.3842.649.
Zotero 插件