硫唑嘌呤对小鼠脾细胞体外核酸和蛋白质合成的抑制作用。
Inhibition of nucleic acid and protein synthesis mouse spleen cells in vitro by azathioprine.
作者信息
Chan G Y, Stone R L
出版信息
Appl Microbiol. 1970 Dec;20(6):910-2. doi: 10.1128/am.20.6.910-912.1970.
Abstract
The effect of azathioprine on macromolecular biosynthesis was studied in mouse spleen cells cultured in vitro. The rate of incorporation of (3)H-thymidine, (3)H-uridine, and (14)C-leucine into acid-insoluble material was used to measure deoxyribonucleic acid, ribonucleic acid, and protein synthesis. Results indicate that azathioprine inhibited nucleic acid and protein synthesis at levels which did not decrease cell viability.
摘要
在体外培养的小鼠脾细胞中研究了硫唑嘌呤对大分子生物合成的影响。用（3）H-胸腺嘧啶核苷、（3）H-尿苷和（14）C-亮氨酸掺入酸不溶性物质的速率来测定脱氧核糖核酸、核糖核酸和蛋白质的合成。结果表明，硫唑嘌呤在不降低细胞活力的水平上抑制核酸和蛋白质合成。
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