小球藻细胞中异柠檬酸裂解酶的消失。
Disappearance of isocitrate lyase enzyme from cells of Chlorella pyrenoidosa.
作者信息
John P C, Thurston C F, Syrett P J
出版信息
Biochem J. 1970 Oct;119(5):913-9. doi: 10.1042/bj1190913.
Abstract
- When acetate-adapted cells of Chlorella are suspended in nitrogen-free medium and supplied with glucose, isocitrate lyase activity disappears from the cells at a rate of about 9%/h. This loss of activity is shown to be accompanied by loss of isocitrate lyase protein. 2. When isocitrate lyase activity is assayed in intact cells after freezing and thawing, the rate of loss of activity after addition of glucose approaches 20%/h. 3. It is shown, by using (35)S, that the rate of turnover of isocitrate lyase protein is somewhat lower than that of other major soluble proteins; general protein turnover during nitrogen starvation, and after glucose addition, is too slow to account for the rate of loss of isocitrate lyase protein. 4. Disappearance of isocitrate lyase activity must result from a mechanism that allows degradation of this specific protein under conditions of limiting nitrogen supply.
摘要
- 当将适应乙酸盐的小球藻细胞悬浮于无氮培养基中并供应葡萄糖时，异柠檬酸裂解酶活性以约9%/小时的速率从细胞中消失。这种活性丧失伴随着异柠檬酸裂解酶蛋白的丧失。2. 当在冻融后的完整细胞中测定异柠檬酸裂解酶活性时，添加葡萄糖后活性丧失速率接近20%/小时。3. 通过使用³⁵S表明，异柠檬酸裂解酶蛋白的周转速率略低于其他主要可溶性蛋白；在氮饥饿期间以及添加葡萄糖后，一般蛋白质周转过于缓慢，无法解释异柠檬酸裂解酶蛋白的丧失速率。4. 异柠檬酸裂解酶活性的消失必定是由一种机制导致的，该机制允许在氮供应受限的条件下降解这种特定蛋白。
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