半胆碱和溴苄铵对离体窦房结自主递质释放的影响。
Effects of hemicholinium and bretylium on the release of autonomic transmitters in the isolated sino-atrial node.
作者信息
Appel W C, Vincenzi F F
出版信息
Br J Pharmacol. 1970 Oct;40(2):268-74. doi: 10.1111/j.1476-5381.1970.tb09920.x.
Abstract
- In the isolated, spontaneously beating, sino-atrial node of the rabbit selective electrical excitation of intranodal autonomic nerve fibres results in a biphasic chronotropic response. This chronotropic response (negative followed by positive chronotropism) is due to the release of the autonomic transmitters (acetylcholine and noradrenaline, respectively) from intranodal nerve fibres.2. In the presence of 2 x 10(-4) g/ml hemicholinium, the negative chronotropic (cholinergic) response is abolished while the positive chronotropic (adrenergic) response is unaltered.3. In the presence of 5 x 10(-6) g/ml bretylium, the positive chronotropic response is abolished while the negative chronotropic response is little affected.4. After blockade of the negative chronotropic response by hemicholinium, bretylium abolishes the remaining positive chronotropic response. The effect of bretylium is not altered in the presence of hemicholinium.5. Considering currently accepted mechanisms of action for hemicholinium and bretylium, the results of these experiments do not lend support to the cholinergic link hypothesis of adrenergic neuro-effector transmission.
摘要
在分离的、自发搏动的兔窦房结中，结内自主神经纤维的选择性电刺激会导致双相变时反应。这种变时反应（负性变时随后是正性变时）是由于结内神经纤维释放自主递质（分别为乙酰胆碱和去甲肾上腺素）所致。
在存在2×10⁻⁴g/ml半胱氨酸的情况下，负性变时（胆碱能）反应被消除，而正性变时（肾上腺素能）反应未改变。
在存在5×10⁻⁶g/ml溴苄铵的情况下，正性变时反应被消除，而负性变时反应受影响较小。
在半胱氨酸阻断负性变时反应后，溴苄铵消除了剩余的正性变时反应。在存在半胱氨酸的情况下，溴苄铵的作用未改变。
考虑到目前公认的半胱氨酸和溴苄铵的作用机制，这些实验结果不支持肾上腺素能神经效应器传递的胆碱能联系假说。
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