坦-霍二氏尿糖蛋白。化学成分。
Tamm-Horsfall urinary glycoprotein. The chemical composition.
作者信息
Fletcher A P, Neuberger A, Ratcliffe W A
出版信息
Biochem J. 1970 Nov;120(2):417-24. doi: 10.1042/bj1200417.
Abstract
- A revised amino acid and carbohydrate composition of human Tamm-Horsfall glycoprotein is presented. 2. No significant differences were obtained in the amino acid composition of Tamm-Horsfall glycoprotein isolated from patients with cystic fibrosis. 3. The glycoprotein was shown to possess a high half-cystine content of 1 per 11-12 amino acid residues, which has been confirmed by performic acid oxidation and S-alkylation with iodoacetate and iodoacetamide. No thiol groups were detected in the glycoprotein. 4. Treatment of the glycoprotein with 0.5m-sodium hydroxide at 4 degrees C for 2 days did not release heterosaccharide material, which suggests that the predominant carbohydrate-protein linkages present are not of the O-glycosidic type. 5. No N-terminal amino acid was detected in the glycoprotein.
摘要
- 本文给出了人Tamm-Horsfall糖蛋白修订后的氨基酸和碳水化合物组成。2. 从囊性纤维化患者中分离出的Tamm-Horsfall糖蛋白，其氨基酸组成没有显著差异。3. 该糖蛋白每11 - 12个氨基酸残基含有1个高半胱氨酸含量，这已通过过甲酸氧化以及用碘乙酸和碘乙酰胺进行S-烷基化得到证实。在该糖蛋白中未检测到巯基。4. 在4℃下用0.5m氢氧化钠处理该糖蛋白2天，未释放出杂糖物质，这表明存在的主要碳水化合物-蛋白质连接不是O-糖苷键类型。5. 在该糖蛋白中未检测到N端氨基酸。
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