视黄醇和芪的黄素光敏反应。
Flavin-photosensitized reactions of retinol and stilbene.
作者信息
Gordon-Walker A, Radda G K
出版信息
Biochem J. 1970 Dec;120(4):673-81. doi: 10.1042/bj1200673e.
Abstract
- Retinol and stilbene are both isomerized when they are illuminated anaerobically in the presence of flavins. 2. Triplet quenchers (e.g. oxygen, potassium iodide and paramagnetic ions) inhibit the reaction more efficiently than they quench flavin fluorescence. At 77 degrees C in a diethyl ether-isopentane-ethanol (5:5:2) glass retinol quenches flavin phosphorescence, but not its fluorescence. 3. For the stilbene reaction cis/trans photostationary-state mixtures are obtained with different flavins and these are linearly related to the phosphorescence transition energies of the flavins used. 4. The reaction involves the triplet state of flavin and a scheme for the reaction is suggested. 5. The dependence of the rate of reaction on substrate concentration is explicable in terms of this scheme. 6. The photobleaching of rhodopsin sensitized by flavin is also demonstrated.
摘要
- 视黄醇和芪在黄素存在下进行厌氧光照时都会发生异构化。2. 三重态猝灭剂（如氧气、碘化钾和顺磁性离子）抑制该反应的效率比猝灭黄素荧光的效率更高。在77摄氏度下，于乙醚 - 异戊烷 - 乙醇（5:5:2）玻璃体系中，视黄醇会猝灭黄素磷光，但不会猝灭其荧光。3. 对于芪反应，使用不同的黄素可得到顺式/反式光稳态混合物，且这些混合物与所用黄素的磷光跃迁能量呈线性相关。4. 该反应涉及黄素的三重态，并提出了一个反应方案。5. 根据此方案可以解释反应速率对底物浓度的依赖性。6. 还证明了黄素敏化的视紫红质的光漂白。
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