一种利用沙海螂心脏测量皮克级乙酰胆碱的简单方法。
A simple method for measuring a picogram of acetylcholine using the clam (Mya arenaria) heart.
作者信息
Cottrell G A, Powell B, Stanton M
出版信息
Br J Pharmacol. 1970 Dec;40(4):866-70. doi: 10.1111/j.1476-5381.1970.tb10661.x.
Abstract
- A simple and reliable method is described for assaying 1 pg or less of acetylcholine (ACh) using a strip of clam (Mya arenaria) heart.2. Clam heart preparations generally are extremely selective for ACh. The preparation described is at least 10(5) times less sensitive to the following: acetyl coenzyme A, adrenaline, choline, dopamine, gamma-aminobutyric acid, glutamic acid, histamine, 5-hydroxytryptamine and noradrenaline.3. Because this preparation is one of the most sensitive known for detecting ACh, it should prove useful for assaying particularly low levels of this substance in biological extracts.
摘要
本文描述了一种使用蛤蜊（砂海螂）心脏条带测定1皮克或更少乙酰胆碱（ACh）的简单可靠方法。
蛤蜊心脏制剂通常对ACh具有极高的选择性。所描述的制剂对以下物质的敏感性至少低10⁵倍：乙酰辅酶A、肾上腺素、胆碱、多巴胺、γ-氨基丁酸、谷氨酸、组胺、5-羟色胺和去甲肾上腺素。
由于该制剂是已知检测ACh最灵敏的制剂之一，它应被证明对测定生物提取物中该物质的极低水平很有用。
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