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一种利用沙海螂心脏测量皮克级乙酰胆碱的简单方法。

A simple method for measuring a picogram of acetylcholine using the clam (Mya arenaria) heart.

作者信息

Cottrell G A, Powell B, Stanton M

出版信息

Br J Pharmacol. 1970 Dec;40(4):866-70. doi: 10.1111/j.1476-5381.1970.tb10661.x.

DOI:10.1111/j.1476-5381.1970.tb10661.x
PMID:5495181
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1702914/
Abstract
  1. A simple and reliable method is described for assaying 1 pg or less of acetylcholine (ACh) using a strip of clam (Mya arenaria) heart.2. Clam heart preparations generally are extremely selective for ACh. The preparation described is at least 10(5) times less sensitive to the following: acetyl coenzyme A, adrenaline, choline, dopamine, gamma-aminobutyric acid, glutamic acid, histamine, 5-hydroxytryptamine and noradrenaline.3. Because this preparation is one of the most sensitive known for detecting ACh, it should prove useful for assaying particularly low levels of this substance in biological extracts.
摘要

  1. 本文描述了一种使用蛤蜊（砂海螂）心脏条带测定1皮克或更少乙酰胆碱（ACh）的简单可靠方法。

  2. 蛤蜊心脏制剂通常对ACh具有极高的选择性。所描述的制剂对以下物质的敏感性至少低10⁵倍：乙酰辅酶A、肾上腺素、胆碱、多巴胺、γ-氨基丁酸、谷氨酸、组胺、5-羟色胺和去甲肾上腺素。

  3. 由于该制剂是已知检测ACh最灵敏的制剂之一，它应被证明对测定生物提取物中该物质的极低水平很有用。

相似文献

1
A simple method for measuring a picogram of acetylcholine using the clam (Mya arenaria) heart.一种利用沙海螂心脏测量皮克级乙酰胆碱的简单方法。
Br J Pharmacol. 1970 Dec;40(4):866-70. doi: 10.1111/j.1476-5381.1970.tb10661.x.
2
A method for concurrent measurement of picomole quantities of acetylcholine, choline, dopamine, norepinephrine, serotonin, 5-hydroxytryptophan, 5-hydroxyindoleacetic acid, tryptophan, tyrosine, glycine, aspartate, glutamate, alanine, and gamma-aminobutyric acid in single tissue samples from different areas of rat central nervous system.一种用于同时测量来自大鼠中枢神经系统不同区域的单个组织样本中皮摩尔量的乙酰胆碱、胆碱、多巴胺、去甲肾上腺素、5-羟色胺、5-羟色氨酸、5-羟吲哚乙酸、色氨酸、酪氨酸、甘氨酸、天冬氨酸、谷氨酸、丙氨酸和γ-氨基丁酸的方法。
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The isolated heart of MYA arenaria as a sensitive preparation for the assay of acetylcholine.作为乙酰胆碱测定的敏感制剂的砂海螂离体心脏。
Br J Pharmacol Chemother. 1955 Mar;10(1):36-8. doi: 10.1111/j.1476-5381.1955.tb00056.x.
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Elapid alpha-toxins have no effect on the cholinergic responses of bivalve myocardia.眼镜蛇科α-毒素对双壳贝类心肌的胆碱能反应没有影响。
Experientia. 1978 Dec 15;34(12):1608-9. doi: 10.1007/BF02034704.

本文引用的文献

1
Measurement of smooth muscle activity in invertebrate animals.无脊椎动物平滑肌活动的测量。
Methods Med Res. 1960;8:187-99.
2
The separation of synaptic vesicles from nerve-ending particles ('synaptosomes').突触小泡与神经末梢颗粒（“突触体”）的分离。
Biochem J. 1964 Feb;90(2):293-303. doi: 10.1042/bj0900293.
3
Is the Mya arenaria heart suitable for ACh bioassay?砂海螂的心脏适合用于乙酰胆碱生物测定吗？
Comp Biochem Physiol. 1968 Dec;27(3):787-94. doi: 10.1016/0010-406x(68)90618-x.