Batrakov S G, Ushakova N A, Gusev M V
Biol Bull Acad Sci USSR. 1979 Jan-Feb;6(1):55-62.
The process of dying of a culture of Str. diacetilactis under conditions of prolonged culturing is accompanied by substantial changes in the composition of the cell lipids. In the lipids of young cells the basic components are mono- and diglucosyldiglycerides, as well as phospholipids--two glucophospholipids, cardiolipin, and phosphatidylglycerin; triglycerides and sterols are present in negligible amounts. As the Str. diacetilactis culture develops and undergoes degradation, an increase is observed in the total amount of extractable lipids. In this case the fraction of phospholipids increases (chiefly on account of cardiolipin), while the fraction of neutral glucolipids decreases. It was shown that in cell homogenates there is a negligible peroxidation of lipids, which is intensified as the culture dies off. There is a parallel acceleration of the uptake of O2 by cell suspensions from cultures of different ages. The detected changes in the lipids are correlated with the morphological and physiological indices of the state of the culture.
在长时间培养条件下，双乙酰乳酸链球菌培养物的死亡过程伴随着细胞脂质组成的显著变化。在年轻细胞的脂质中，基本成分是单葡萄糖二甘油酯和二葡萄糖二甘油酯，以及磷脂——两种葡萄糖磷脂、心磷脂和磷脂酰甘油；甘油三酯和固醇的含量极少。随着双乙酰乳酸链球菌培养物的生长和降解，可提取脂质的总量增加。在这种情况下，磷脂的比例增加（主要是由于心磷脂），而中性糖脂的比例下降。结果表明，在细胞匀浆中脂质的过氧化作用可忽略不计，随着培养物死亡，过氧化作用加剧。不同年龄培养物的细胞悬液对氧气的摄取也同时加速。检测到的脂质变化与培养物状态的形态学和生理学指标相关。
