Ashurst S W, Cohen G M
Chem Biol Interact. 1979 Dec;28(2-3):279-89. doi: 10.1016/0009-2797(79)90168-6.
Five distinct hydrocarbon-deoxyribonucleoside adducts are separated by high pressure liquid chromatography after reaction of benzo[alpha]pyrene with calf thymus DNA in the presence of liver microsomes from 3-methylcholanthrene treated rats. The two major adducts co-chromatography with deoxyribonucleoside adducts obtained after hydrolysis of calf thymus DNA previously reacted with liver microsomal metabolically activated 9-hydroxy-benzo[alpha]pyrene or trans-7,8-dihydro-7,8-dihydroxybenzo[alpha]pyrene. High magnesium ion concentrations in the microsomal incubations cause a significant decrease in the covalent binding of the hydrocarbon to DNA but do not affect the qualitative distribution of the individual benzo[alpha]pyrene-deoxyribonucleoside adducts.
在来自经3-甲基胆蒽处理的大鼠的肝微粒体存在的情况下，苯并[a]芘与小牛胸腺DNA反应后，通过高压液相色谱法分离出五种不同的烃-脱氧核糖核苷加合物。这两种主要加合物与小牛胸腺DNA预先与肝微粒体代谢活化的9-羟基苯并[a]芘或反式-7,8-二氢-7,8-二羟基苯并[a]芘反应后水解得到的脱氧核糖核苷加合物共色谱。微粒体孵育中高镁离子浓度导致烃与DNA的共价结合显著降低，但不影响各个苯并[a]芘-脱氧核糖核苷加合物的定性分布。
