Evans R H, Haynes J, Morris C J, Woolf A L
J Neurol Neurosurg Psychiatry. 1970 Dec;33(6):783-5. doi: 10.1136/jnnp.33.6.783.
A method is described for vital staining of the human intramuscular nerve endings after the muscle biopsy has been removed from the patient. The muscle is transferred from the site of operation to the laboratory in oxygenated physiological saline. Methylene blue solution (0·015%) is injected into the muscle strip, and development of the stain can be observed under the microscope after exposure of the muscle to air. Permanent squash preparations can be made of the stained muscle and these are identical in appearance to muscles stained by injection of methylene blue in vivo.
本文描述了一种在从患者身上取下肌肉活检标本后对人肌肉内神经末梢进行活体染色的方法。将肌肉从手术部位转移至装有充氧生理盐水的实验室。将亚甲蓝溶液（0.015%）注入肌肉条，在肌肉暴露于空气后，可在显微镜下观察染色的显色情况。可以对染色后的肌肉制作永久性压片标本，其外观与通过体内注射亚甲蓝染色的肌肉相同。
