腺病毒12诱导的仓鼠肿瘤体外转移核糖核酸甲基酶活性的特性。碱基分析。
Properties of the transfer ribonucleic acid methylase activity in vitro of hamster tumours induced by adenovirus-12. Base analysis.
作者信息
McFarlane E S, Lee C G
出版信息
Biochem J. 1970 Dec;120(3):499-503. doi: 10.1042/bj1200499.
Abstract
The tRNA methylase activity in vitro of adenovirus-12-induced tumour and normal hamster tissues was studied. Enzyme extracts from adenovirus-12-induced tumour, hamster muscle-connective tissue and hamster liver methylated both Escherichia coli tRNA and yeast tRNA. The source of the enzyme and the pH of the incubation mixture influenced the bases methylated. However, the methylated bases that were isolated were not affected by the presence of NH(4) (+) ions in the incubation mixture.
摘要
对腺病毒12诱导的肿瘤组织和正常仓鼠组织的tRNA甲基化酶体外活性进行了研究。从腺病毒12诱导的肿瘤组织、仓鼠肌肉结缔组织和仓鼠肝脏中提取的酶提取物，可使大肠杆菌tRNA和酵母tRNA甲基化。酶的来源和孵育混合物的pH值会影响甲基化的碱基。然而，分离得到的甲基化碱基不受孵育混合物中NH(4) (+)离子存在的影响。
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