酵母乙醇脱氢酶对二氢烟酰胺-腺嘌呤二核苷酸和吡啶-3-醛-腺嘌呤二核苷酸的结合作用
The binding of dihydronicotinamide--adenine dinucleotide and pyridine-3-aldehyde--adenine dinucleotide by yeast alcohol dehydrogenase.
作者信息
Dickinson F M
出版信息
Biochem J. 1970 Dec;120(4):821-30. doi: 10.1042/bj1200821.
Abstract
- Yeast alcohol dehydrogenase has been found to react with NADH in the presence of acetamide to form a highly fluorescent ternary complex. Titration of the enzyme to form this complex has provided a method for the estimation of the number of binding sites on the enzyme. 2. The binding of NADH by the enzyme has been studied independently, with a modified form of equilibrium dialysis, by using gel filtration. 3. A third method, depending upon the formation of a ternary complex of enzyme, hydroxylamine and pyridine-3-aldehyde-adenine dinucleotide, has also been used to titrate the enzyme. 4. Values obtained with all three methods are substantially in agreement that only three coenzyme-binding sites are available. This is in contrast with the established fact that the enzyme is composed of four identical subunits.
摘要
- 已发现酵母乙醇脱氢酶在乙酰胺存在下与NADH反应形成高度荧光的三元复合物。滴定该酶以形成此复合物提供了一种估算酶上结合位点数量的方法。2. 已通过使用凝胶过滤，采用改良形式的平衡透析法独立研究了该酶与NADH的结合。3. 还使用了第三种方法，该方法依赖于酶、羟胺和吡啶 - 3 - 醛 - 腺嘌呤二核苷酸形成三元复合物来滴定该酶。4. 用这三种方法获得的值基本一致，即只有三个辅酶结合位点可用。这与该酶由四个相同亚基组成这一既定事实形成对比。
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Some observations on the preparation and properties of dihydronicotinamide-adenine dinucleotide.关于二氢烟酰胺腺嘌呤二核苷酸制备及性质的一些观察
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