用硝基蓝四氮唑（NBT）和维生素E（VE）抑制绵羊精囊微粒体（SVGM）中前列腺素（PG）的合成。

Inhibition of prostaglandin (PG) synthesis in sheep vesicular gland microsomes (SVGM) by nitroblue tetrazolium (NBT) and vitamin E (VE).

作者信息

Rao G H, Burris S M, Gerrard J M, White J G

出版信息

Prostaglandins Med. 1979 Feb;2(2):111-21. doi: 10.1016/0161-4630(79)90046-6.

Abstract

Previous studies have shown that NBT and VE together are potent inhibitors of platelet aggregation, secretion and PG synthesis. In this study, we evaluated the capacity of NBT to detect PG synthesis by SVGM. Aspirin pretreatment of SVGM decreased the amount of NBT reduced after addition of arachidonic acid, demonstrating that products generated by the cyclo-oxygenase were involved in NBT reduction. The influence of NBT and VE on PG synthesis by SVGM was then evaluated by measuring malondialdehyde (MDA) production. NBT or VE alone had no significant effect, but together these agents were as effective as aspirin in preventing MDA formation. The effect of NBT and VE on 14C-arachidonic acid conversion was followed by thin layer chromatography and radioscanning. Again, NBT or VE alone were ineffective, whereas the combination was as effective as aspirin in preventing conversion of arachidonic acid. We speculate NBT and VE together inhibit pg synthesis by scavenging a free radical species of arachidonic acid generated in the initial step of fatty acid peroxidation.

摘要

先前的研究表明，NBT和VE共同作用时是血小板聚集、分泌及PG合成的有效抑制剂。在本研究中，我们评估了NBT检测SVGM合成PG的能力。对SVGM进行阿司匹林预处理后，添加花生四烯酸后NBT还原量减少，表明环氧化酶产生的产物参与了NBT的还原过程。然后通过测量丙二醛（MDA）生成量来评估NBT和VE对SVGM合成PG的影响。单独使用NBT或VE没有显著影响，但二者共同作用时在防止MDA形成方面与阿司匹林效果相同。通过薄层色谱法和放射扫描追踪NBT和VE对14C-花生四烯酸转化的影响。同样，单独使用NBT或VE无效，而二者组合在防止花生四烯酸转化方面与阿司匹林效果相同。我们推测NBT和VE共同作用通过清除脂肪酸过氧化初始步骤中产生的花生四烯酸自由基来抑制PG合成。