体外培养的小脑颗粒细胞。光镜与电镜研究
Cerebellar granule cells in vitro. A light and electron microscope study.
作者信息
Seil F J, Herndon R M
出版信息
J Cell Biol. 1970 May;45(2):212-20. doi: 10.1083/jcb.45.2.212.
Abstract
The behavior of granule cells in mature cerebellar cultures derived from newborn mice was studied by light and electron microscopy. Many granule cells remained in the explants as an external granular layer. These cells were differentiated, as evidenced by formation of bundles of parallel fibers and by development of synapses between granule cell axons and Purkinje cell branchlet spines, and between Golgi cell axons and granule cell dendrites. Although the over-all architecture of the cerebellar explants after 18-33 days in vitro was similar to that of the newborn mouse, the evident differentiation of the granule cells suggested that interneuronal relationships resemble those of the mature cerebellum in vivo.
摘要
通过光学显微镜和电子显微镜研究了新生小鼠来源的成熟小脑培养物中颗粒细胞的行为。许多颗粒细胞作为外颗粒层留在外植体中。这些细胞已分化，平行纤维束的形成以及颗粒细胞轴突与浦肯野细胞分支棘之间、高尔基细胞轴突与颗粒细胞树突之间突触的发育证明了这一点。尽管体外培养18 - 33天后小脑外植体的整体结构与新生小鼠相似，但颗粒细胞的明显分化表明神经元间关系类似于体内成熟小脑的神经元间关系。
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