Hermann G, Hermann M, Neubert K, Hübner G, Barth A
Acta Biol Med Ger. 1979;38(11-12):1509-19.
The relatively alanine-specific amino peptidase from Brassica napus hydrolyzes dipeptidyl-4-(phenylazo)-phenylamide according to a consecutive mechanism. Enzyme-kinetic studies of dipeptidyl-4-(phenylazo)-phenylamides carried out by means of spectrometrical procedures of measurement are only possible when the increase in the concentration of the fission product p-aminoazobenzene is recorded. For assessing the kinetic parameters of the intermediary steps of the hydrolysis of dipeptidyl-4-(phenylazo)-phenylamides a function is derived describing the dependence of the concentration of fission products of p-aminoazobenzene on reaction time. The satisfactory correspondence of the kinetic parameters calculated for the intermediary-originating substrate L-ala-4-(phenylazo)-phenylamide as substrate demonstrates the applicability of the procedure described in the present paper.
来自甘蓝型油菜的相对丙氨酸特异性氨肽酶按照连续机制水解二肽基-4-(苯偶氮)-苯酰胺。只有当记录到裂变产物对氨基偶氮苯浓度的增加时，通过光谱测量程序对二肽基-4-(苯偶氮)-苯酰胺进行酶动力学研究才是可行的。为了评估二肽基-4-(苯偶氮)-苯酰胺水解中间步骤的动力学参数，推导了一个描述对氨基偶氮苯裂变产物浓度与反应时间依赖性的函数。以中间产物L-丙氨酸-4-(苯偶氮)-苯酰胺为底物计算得到的动力学参数的良好对应性证明了本文所述方法的适用性。
