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在氨基酸分析仪上使用自动色谱法,采用柠檬酸锂缓冲液分离核酸碱基、核苷、核苷酸及其前体。

Use of automated chromatography on the amino acid analyzer with lithium citrate buffers to separate nucleic acid bases, nucleosides, nucleotides and their precursors.

作者信息

Tyagi A K, Jayaram H N, Anandaraj S, Taylor B, Cooney D A

出版信息

J Biochem Biophys Methods. 1979 Aug;1(4):221-6. doi: 10.1016/0165-022x(79)90008-3.

DOI:10.1016/0165-022x(79)90008-3
PMID:552388
Abstract

A simple and sensitive method has been developed to separate nucleic acid bases, nucleosides, nucleotides and their precursors by automated chromatography using the amino acid analyzer with lithium citrate buffers. The method is sensitive to a concentration of 5 nmol, linear in the range of 5--100 nmol, and resolves almost all the bases, nucleosides, nucleotides and their precursors of physiologic importance.

摘要

已开发出一种简单且灵敏的方法,可使用配备柠檬酸锂缓冲液的氨基酸分析仪,通过自动色谱法分离核酸碱基、核苷、核苷酸及其前体。该方法对5纳摩尔的浓度敏感,在5 - 100纳摩尔范围内呈线性,并且几乎能分离所有具有生理重要性的碱基、核苷、核苷酸及其前体。

相似文献

1
Use of automated chromatography on the amino acid analyzer with lithium citrate buffers to separate nucleic acid bases, nucleosides, nucleotides and their precursors.在氨基酸分析仪上使用自动色谱法,采用柠檬酸锂缓冲液分离核酸碱基、核苷、核苷酸及其前体。
J Biochem Biophys Methods. 1979 Aug;1(4):221-6. doi: 10.1016/0165-022x(79)90008-3.
2
Separation of nucleosides and nucleotides by reversed-phase high-performance liquid chromatography with volatile buffers allowing sample recovery.使用挥发性缓冲液通过反相高效液相色谱法分离核苷和核苷酸,实现样品回收。
Anal Biochem. 1985 May 15;147(1):180-5. doi: 10.1016/0003-2697(85)90025-9.
3
Cation-exchange chromatography of nucleotides, nucleosides and nucleic bases.核苷酸、核苷和核酸碱基的阳离子交换色谱法。
J Chromatogr. 1971 Dec 23;53(3):584-6. doi: 10.1016/s0021-9673(01)98519-x.
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Extraction and measurement of myocardial nucleotides, nucleosides, and purine bases by high-performance liquid chromatography.
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Rapid preparation of nucleotides from acid-soluble pools by chromatography on silica, as exemplified with acid extracts of cultured cells.通过硅胶柱色谱法从酸溶性组分中快速制备核苷酸,以培养细胞的酸提取物为例。
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6
PEI-cellulose thin-layer chromatography: a highly versatile system for separating purine and pyrimidine nucleotides from nucleosides and free bases.聚乙撑亚胺纤维素薄层层析法:一种用于从核苷和游离碱基中分离嘌呤和嘧啶核苷酸的高度通用的系统。
Anal Biochem. 1972 Nov;50(1):35-9. doi: 10.1016/0003-2697(72)90481-2.
7
Accelerated amino acid analysis: studies on the use of lithium citrate buffers and the effect of n-propanol, in the analysis of physiological fluids and protein hydrolyzates.加速氨基酸分析:关于在生理体液和蛋白质水解产物分析中使用柠檬酸锂缓冲液及正丙醇影响的研究。
Anal Biochem. 1970 Dec;38(2):313-29. doi: 10.1016/0003-2697(70)90456-2.
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Separation of nucleic acid bases, nucleosides and nucleotides on strong cation-exchange thin layers. IX. Separation of cyclic nucleotides.强阳离子交换薄层上核酸碱基、核苷及核苷酸的分离。IX. 环核苷酸的分离。
J Chromatogr. 1979 Feb 1;169:466-8. doi: 10.1016/0021-9673(75)85084-9.
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Rapid separation of nucleosides and nucleotides by cation-exchange column chromatography.通过阳离子交换柱色谱法快速分离核苷和核苷酸。
J Chromatogr. 1969 Oct 28;44(2):342-8. doi: 10.1016/s0021-9673(01)92545-2.
10
Automated micro procedures for peptide separations.用于肽分离的自动化微量程序。
Methods Enzymol. 1977;47:220-36. doi: 10.1016/0076-6879(77)47027-7.