Tyagi A K, Jayaram H N, Anandaraj S, Taylor B, Cooney D A
J Biochem Biophys Methods. 1979 Aug;1(4):221-6. doi: 10.1016/0165-022x(79)90008-3.
A simple and sensitive method has been developed to separate nucleic acid bases, nucleosides, nucleotides and their precursors by automated chromatography using the amino acid analyzer with lithium citrate buffers. The method is sensitive to a concentration of 5 nmol, linear in the range of 5--100 nmol, and resolves almost all the bases, nucleosides, nucleotides and their precursors of physiologic importance.
已开发出一种简单且灵敏的方法,可使用配备柠檬酸锂缓冲液的氨基酸分析仪,通过自动色谱法分离核酸碱基、核苷、核苷酸及其前体。该方法对5纳摩尔的浓度敏感,在5 - 100纳摩尔范围内呈线性,并且几乎能分离所有具有生理重要性的碱基、核苷、核苷酸及其前体。