Khaĭsman E B, Alekseev O V
Biull Eksp Biol Med. 1976 Jan;81(1):81-4.
The work was devoted to the ultrastructural analysis of the neurohistological preparations. Sections of the tissue from the precardial parts of the pulmonary and caval dog veins were impregnated with silver salts after Campos and embedded in the araldite by a special method. Electronmicroscopi studies showed reduced silver adsorbed by the tissue of the impregnated preparations to exhibit a granular structure (the granules were 30-400 A in size). The largest of them were revealed in the axoplasm of the myelinated and unmyelinated nerve conductors, whereas the smaller ones found in various cellular and fibrous formations of the tissue substrate; silver granules were as a rule absent within the thickness of the myelin sheath. The noted regularities of adsorption and distribution of the silver granules in the impregnated preparations caused a morphologically expressed phenomenon of argentophilia.
这项工作致力于对神经组织学制剂进行超微结构分析。按照坎波斯的方法,用银盐对取自犬肺静脉和腔静脉心前区部分的组织切片进行浸染,然后用一种特殊方法将其包埋在环氧树脂中。电子显微镜研究显示,浸染制剂的组织吸附的银减少,呈现出颗粒结构(颗粒大小为30 - 400埃)。其中最大的颗粒出现在有髓和无髓神经传导器的轴浆中,而较小的颗粒则存在于组织基质的各种细胞和纤维结构中;髓鞘厚度内通常没有银颗粒。在浸染制剂中银颗粒吸附和分布的上述规律导致了一种在形态学上表现明显的嗜银现象。