从鸡蛋中分离和鉴定两种磷蛋白。
The isolation and compsotion of two phosphoproteins from hen's egg.
作者信息
Clark R C
出版信息
Biochem J. 1970 Jul;118(3):537-42. doi: 10.1042/bj1180537.
Abstract
- Phosvitin extracted from domestic hen's-egg yolk was resolved on Sephadex G-100 into two phosphoprotein components. 2. The major component has a molecular weight of about 3.4x10(4) and alanine as an N-terminal residue. Glucosamine is present, but tyrosine is virtually absent. 3. The minor component has a molecular weight of about 2.8x10(4) and lysine as an N-terminal residue. Missing residues are glucosamine, methionine and leucine. Lysine, histidine, threonine, glycine, phenylalanine and tyrosine contents differ significantly from those of the major component. 4. Sephadex G-100 also removes small amounts of an impurity with a much higher molecular weight.
摘要
- 从家鸡蛋黄中提取的卵黄高磷蛋白在葡聚糖凝胶G - 100上分离为两种磷蛋白成分。2. 主要成分的分子量约为3.4×10⁴，N端残基为丙氨酸。含有氨基葡萄糖，但几乎不含酪氨酸。3. 次要成分的分子量约为2.8×10⁴，N端残基为赖氨酸。缺少的残基是氨基葡萄糖、蛋氨酸和亮氨酸。赖氨酸、组氨酸、苏氨酸、甘氨酸、苯丙氨酸和酪氨酸的含量与主要成分有显著差异。4. 葡聚糖凝胶G - 100还能去除少量分子量高得多的杂质。
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