Hearing V J, Nicholson J M
Cancer Biochem Biophys. 1979;4(2):59-68.
Abnormal proteins in neoplastic cells are present in a variety of animal tumor systems. Our laboratory has isolated several of these abnormal proteins from murine malignant melanoma by preparative gel electrophoresis; these proteins are similar in many respects to analogous proteins found in normal tissues. Examination of these proteins by polyacrylamide gel electrophoresis, amino acid analysis, carboxy and amino terminal analysis and peptide mapping after cyanogen bromide cleavage, supports the theory that these abnormal proteins are the result of deleted sequences in the peptide chain during the attempted synthesis of normal proteins in neoplastic tissue.
肿瘤细胞中的异常蛋白质存在于多种动物肿瘤系统中。我们实验室通过制备性凝胶电泳从鼠恶性黑色素瘤中分离出了几种此类异常蛋白质；这些蛋白质在许多方面与正常组织中发现的类似蛋白质相似。通过聚丙烯酰胺凝胶电泳、氨基酸分析、羧基和氨基末端分析以及溴化氰裂解后的肽图谱分析这些蛋白质，支持了这样一种理论，即这些异常蛋白质是肿瘤组织中正常蛋白质合成尝试过程中肽链中序列缺失的结果。
