Mayfield J E, Ellison J R
Chromosoma. 1976 Jan 27;54(1):27-31. doi: 10.1007/BF00331830.
Nuclei from various mouse tissues exhibit a pattern of fluorescence characteristic of the cell type when stained with the fluorescent compound Hoechst 33258. When such preparations are hybridized in situ with 3H-RNA complementary to the A-T rich satellite of mouse, it is clearly seen that only the fluorescent regions of the nuclei contain the satellite DNA. Thus Hoechst 33258 allows the precise localization of satellite DNA at all stages of the mouse cell cycle.
当用荧光化合物赫斯特33258染色时，来自各种小鼠组织的细胞核呈现出该细胞类型特有的荧光模式。当这些标本与与小鼠富含A - T的卫星DNA互补的3H - RNA进行原位杂交时，可以清楚地看到只有细胞核的荧光区域含有卫星DNA。因此，赫斯特33258能在小鼠细胞周期的所有阶段精确地定位卫星DNA。
