[某些洗涤剂胶束对蛋白质滴定的干扰]
[Interference of some detergent micelles with the titration of protein].
作者信息
Bruscalupi G, Leoni S, Panzali A F, Spagnuolo S, Vullo G
出版信息
Boll Soc Ital Biol Sper. 1979 Mar 15;55(5):450-4.
PMID:553600
Abstract
We have examined the possible interferences with spectrophotometric protein determination of some detergents commonly used for the solubilization of membrane proteins. The results show that some mixtures of Triton X100 with SDS or DOC, at given concentrations, interfere with BSA determination by the method of Lowry.
摘要
我们已经研究了一些常用于溶解膜蛋白的去污剂对分光光度法测定蛋白质的可能干扰。结果表明，在给定浓度下，Triton X100与SDS或DOC的某些混合物会干扰用Lowry法测定牛血清白蛋白。
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