Corcelli A, Storelli-Joss C, Lippe C, Storelli C
Boll Soc Ital Biol Sper. 1979 Mar 15;55(5):460-6.
L-lactate uptake was measured in vesicles formed by intestinal brush border and baso-lateral membranes, using a rapid filtration technique. In the presence of a Na+ gradient directed into the vesicle, L-lactate can be transiently accumulated in brush border vesicles, but not in baso-lateral ones. The transient L-lactate accumulation does not occur in the presence of a KCl gradient. alpha-cyanocinammic acid strongly inhibits L-lactate uptake in brush border vesicles, but not in baso-lateral ones. These results support the existence of a carrier mediated, Na+ dependent, transport of L-lactate across the brush border membrane.
采用快速过滤技术，测定了由肠刷状缘膜和基底外侧膜形成的囊泡对L-乳酸的摄取。在存在指向囊泡内部的Na⁺梯度的情况下，L-乳酸可在刷状缘囊泡中短暂积累，但在基底外侧囊泡中则不会。在存在KCl梯度的情况下，不会发生L-乳酸的短暂积累。α-氰基肉桂酸强烈抑制刷状缘囊泡对L-乳酸的摄取，但不抑制基底外侧囊泡的摄取。这些结果支持存在一种载体介导的、依赖Na⁺的L-乳酸跨刷状缘膜的转运。
