大肠杆菌B-r中araC基因温度敏感突变体中L-阿拉伯糖操纵子表达的调控
Control of expression of the L-arabinose operon in temperature-sensitive mutants of gene araC in Escherichia coli B-r.
作者信息
Irr J, Englesberg E
出版信息
J Bacteriol. 1971 Jan;105(1):136-41. doi: 10.1128/jb.105.1.136-141.1971.
Abstract
Expression of the l-arabinose operon in Escherichia coli B/r is dependent on the temperature of growth of the araC mutants reported in this paper. Analysis of these temperature-sensitive regulatory mutants indicates that both repressor and activator activities are thermolabile. The simplest model to explain the manner in which the operon is controlled is one suggesting that the regulatory gene, araC, codes for a protein which upon synthesis acts as a repressor molecule and prevents operon function. When inducer is added, the repressor undergoes a conformational shift and becomes an activator which switches on enzyme synthesis, provided the repressor concentration is reduced to a sufficiently low level in the cell. These data lend strong support to the model that both activities are the result of the same gene product.
摘要
本文报道的大肠杆菌B/r中L-阿拉伯糖操纵子的表达取决于araC突变体的生长温度。对这些温度敏感型调控突变体的分析表明，阻遏物和激活物活性均不耐热。解释该操纵子调控方式的最简单模型表明，调控基因araC编码一种蛋白质，该蛋白质在合成后作为阻遏分子发挥作用并阻止操纵子功能。当加入诱导剂时，阻遏物发生构象转变并成为激活物，从而开启酶的合成，前提是细胞中阻遏物浓度降低到足够低的水平。这些数据有力地支持了这一模型，即两种活性均为同一基因产物的结果。
相似文献
1
Control of expression of the L-arabinose operon in temperature-sensitive mutants of gene araC in Escherichia coli B-r.大肠杆菌B-r中araC基因温度敏感突变体中L-阿拉伯糖操纵子表达的调控
J Bacteriol. 1971 Jan;105(1):136-41. doi: 10.1128/jb.105.1.136-141.1971.
2
Dominance relationships among mutant alleles of regulatory gene araC in the Escherichia coli B/R L-arabinose operon.大肠杆菌B/R L-阿拉伯糖操纵子中调控基因araC突变等位基因之间的显性关系。
J Bacteriol. 1986 Nov;168(2):999-1001. doi: 10.1128/jb.168.2.999-1001.1986.
3
Regulatory properties of araC(c) mutants in the L-arabinose operon of escherichia coliB/r.大肠杆菌B/r中L-阿拉伯糖操纵子中araC(c)突变体的调控特性
J Bacteriol. 1978 Jan;133(1):178-84. doi: 10.1128/jb.133.1.178-184.1978.
4
D-Fucose as a gratuitous inducer of the L-arabinose operon in strains of Escherichia coli B-r mutant in gene araC.D-岩藻糖作为阿拉伯糖操纵子在大肠杆菌B-r araC基因突变株中的 gratuitous诱导剂 。 （注：这里“gratuitous”不太明确准确意思，可根据具体专业背景进一步确认准确含义来完善译文表述）
J Bacteriol. 1971 Jul;107(1):79-86. doi: 10.1128/jb.107.1.79-86.1971.
5
Negative regulation of L-arabinose metabolism in Bacillus subtilis: characterization of the araR (araC) gene.枯草芽孢杆菌中L-阿拉伯糖代谢的负调控：araR（araC）基因的特性分析
J Bacteriol. 1997 Mar;179(5):1598-608. doi: 10.1128/jb.179.5.1598-1608.1997.
6
Interaction between mutant alleles of araC of the Escherichia coli B/r L-arabinose operon.大肠杆菌B/r L-阿拉伯糖操纵子araC突变等位基因之间的相互作用。
J Bacteriol. 1979 Sep;139(3):1085-8. doi: 10.1128/jb.139.3.1085-1088.1979.
7
Mutations in the araC regulatory gene of Escherichia coli B/r that affect repressor and activator functions of AraC protein.大肠杆菌B/r的araC调控基因中的突变，这些突变影响AraC蛋白的阻遏物和激活物功能。
J Bacteriol. 1986 Jun;166(3):892-900. doi: 10.1128/jb.166.3.892-900.1986.
8
Transcriptional control in the L-arabinose operon of Escherichia coli B-r.大肠杆菌B-r的L-阿拉伯糖操纵子中的转录调控
J Bacteriol. 1974 Apr;118(1):121-8. doi: 10.1128/jb.118.1.121-128.1974.
9
Arabinose-leucine deletion mutants of Escherichia coli B-r.大肠杆菌B-r的阿拉伯糖-亮氨酸缺失突变体
J Bacteriol. 1969 Jun;98(3):1159-69. doi: 10.1128/jb.98.3.1159-1169.1969.
10
L-Arabinose-sensitive, L-ribulose 5-phosphate 4-epimerase-deficient mutants of Escherichia coli.大肠杆菌对L-阿拉伯糖敏感、缺乏5-磷酸-L-核酮糖4-差向异构酶的突变体
J Bacteriol. 1962 Jul;84(1):137-46. doi: 10.1128/jb.84.1.137-146.1962.
引用本文的文献
1
Temperature sensitive mutants affecting the activity and regulation of the acetamidase of Aspergillus nidulans.影响构巢曲霉乙酰胺酶活性及调控的温度敏感突变体。
Mol Gen Genet. 1973 Jul 16;123(4):333-46. doi: 10.1007/BF00433650.
2
Genetic control of enzyme induction in the -ketoadipate pathway of Pseudomonas putida: deletion mapping of cat mutations.恶臭假单胞菌α-酮己二酸途径中酶诱导的遗传控制：cat突变的缺失定位
J Bacteriol. 1972 Feb;109(2):790-5. doi: 10.1128/jb.109.2.790-795.1972.
3
L-arabinose isomerase formation in a conditional mutant of gene araA of Escherichia coli B-r.大肠杆菌B-r基因araA的条件突变体中L-阿拉伯糖异构酶的形成
J Bacteriol. 1972 Dec;112(3):1247-53. doi: 10.1128/jb.112.3.1247-1253.1972.
4
Genetic control of the 2-keto-3-deoxy-d-gluconate metabolism in Escherichia coli K-12: kdg regulon.大肠杆菌K-12中2-酮-3-脱氧-d-葡萄糖酸代谢的遗传控制：kdg调节子
J Bacteriol. 1974 Feb;117(2):641-51. doi: 10.1128/jb.117.2.641-651.1974.
5
Positive regulation of amidase synthesis in Pseudomonas aeruginosa.铜绿假单胞菌中酰胺酶合成的正调控
J Bacteriol. 1978 Aug;135(2):379-92. doi: 10.1128/jb.135.2.379-392.1978.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
L-ARABINOSE NEGATIVE MUTANTS OF THE L-RIBULOKINASE STRUCTURAL GENE AFFECTING THE LEVELS OF L-ARABINOSE ISOMERASE IN ESCHERICHIA COLI.影响大肠杆菌中L-阿拉伯糖异构酶水平的L-核糖激酶结构基因的L-阿拉伯糖阴性突变体
Genetics. 1964 Jan;49(1):95-108. doi: 10.1093/genetics/49.1.95.
3
COORDINATE VARIATIONS IN INDUCED SYNTHESES OF ENZYMES ASSOCIATED WITH MUTATIONS IN A STRUCTURAL GENE.与结构基因突变相关的酶诱导合成中的坐标变化。
Proc Natl Acad Sci U S A. 1963 Oct;50(4):696-702. doi: 10.1073/pnas.50.4.696.
4
Determination of the order of mutational sites governing L-arabinose utilization in Escherichia coli B/r bv transduction with phage Plbt.用噬菌体Plbt对大肠杆菌B/r bv进行转导时，确定控制L-阿拉伯糖利用的突变位点顺序
Virology. 1959 Nov;9:314-31. doi: 10.1016/0042-6822(59)90125-4.
5
Positive control of transcription by a bacteriophage sigma factor.噬菌体σ因子对转录的正调控
Nature. 1970 Mar 14;225(5237):1009-12. doi: 10.1038/2251009a0.
6
Further evidence for positive control of the L-arabinose system by gene araC.基因araC对L-阿拉伯糖系统进行正向调控的进一步证据。
J Mol Biol. 1967 May 14;25(3):443-54. doi: 10.1016/0022-2836(67)90197-0.
7
Positive control of enzyme synthesis by gene C in the L-arabinose system.在L-阿拉伯糖系统中，基因C对酶合成的正调控。
J Bacteriol. 1965 Oct;90(4):946-57. doi: 10.1128/jb.90.4.946-957.1965.
8
Nonsense mutants in the regulator gene araC of the L-arabinose system of Escherichia coli B-r.大肠杆菌B-r的L-阿拉伯糖系统调节基因araC中的无义突变体。
Genetics. 1970 May;65(1):27-39. doi: 10.1093/genetics/65.1.27.
9
Separation and characterization of the subunits of ribonucleic acid polymerase.核糖核酸聚合酶亚基的分离与特性分析
J Biol Chem. 1969 Nov 25;244(22):6168-76.
10
An analysis of "revertants" of a deletion mutant in the C gene of the L-arabinose gene complex in Escherichia coli B-r: isolation of initiator constitutive mutants (Ic).大肠杆菌B-r中L-阿拉伯糖基因复合体C基因缺失突变体“回复体”的分析：起始组成型突变体（Ic）的分离
J Mol Biol. 1969 Jul 28;43(2):281-98. doi: 10.1016/0022-2836(69)90268-x.
Zotero 插件