Molina J A, Alexander M
J Bacteriol. 1971 Feb;105(2):489-93. doi: 10.1128/jb.105.2.489-493.1971.
Extracts of the hyphae of a nitrifying strain of Aspergillus flavus formed nitrite and nitrate from 3-nitropropionate. Nicotinamide adenine dinucleotide phosphate and nicotinamide adenine dinucleotide enhanced the production of nitrate but not nitrite, whereas cysteine and diethyldithiocarbamate increased nitrite but diminished nitrate synthesis. Quinacrine reduced the extent of conversion of the nitro compound to nitrite and nitrate, but only the inhibition of nitrite formation was completely reversed by flavine coenzymes. Molecular oxygen was essential for this part of the nitrification sequence. 3-Chloropropionate stimulated the oxidation of nitrite by hyphae or enzyme preparations. Although the fungus contained a noncytochrome-linked nitrite-oxidizing enzyme, partially purified preparations free of this enzyme formed both nitrite and nitrate from 3-nitropropionate. Possible mechanisms of this latter stage of heterotrophic nitrification are discussed.
黄曲霉硝化菌株的菌丝提取物可将3-硝基丙酸转化为亚硝酸盐和硝酸盐。烟酰胺腺嘌呤二核苷酸磷酸和烟酰胺腺嘌呤二核苷酸可促进硝酸盐的生成，但对亚硝酸盐的生成无促进作用，而半胱氨酸和二乙基二硫代氨基甲酸盐则增加了亚硝酸盐的生成，但减少了硝酸盐的合成。奎纳克林降低了硝基化合物向亚硝酸盐和硝酸盐的转化程度，但只有亚硝酸盐生成的抑制作用能被黄素辅酶完全逆转。分子氧对于硝化序列的这一部分至关重要。3-氯丙酸可刺激菌丝或酶制剂对亚硝酸盐的氧化。尽管该真菌含有一种非细胞色素连接的亚硝酸盐氧化酶，但不含该酶的部分纯化制剂也能将3-硝基丙酸转化为亚硝酸盐和硝酸盐。本文讨论了异养硝化这一后期阶段的可能机制。
