Rebeiz C A, Castelfranco P A
Plant Physiol. 1971 Jan;47(1):33-7. doi: 10.1104/pp.47.1.33.
A crude homogenate obtained from greening cucumber (Cucumis sativus, L.) cotyledons in tris-sucrose, pH 7.7, containing coenzyme A, glutathione, potassium phosphate, pH 7.7, methyl alcohol, magnesium, nicotinamide adenine dinucleotide, and adenosine triphosphate, is able to incorporate 4-(14)C-delta-aminolevulinic acid into chlorophyll a and b in the presence of oxygen. If the homogenates are prepared from etiolated cotyledons which have been exposed to light for two and one-half hours, (14)C-chlorophyll a is synthesized. However, when the homogenates are prepared from cotyledons illuminated for four and one-half hours, both (14)C-chlorophyll a and b are produced.
从处于变绿阶段的黄瓜(黄瓜属,L.)子叶在pH 7.7的三羟甲基氨基甲烷 - 蔗糖溶液中制备的粗匀浆,含有辅酶A、谷胱甘肽、pH 7.7的磷酸钾、甲醇、镁、烟酰胺腺嘌呤二核苷酸和三磷酸腺苷,在有氧气存在的情况下能够将4-(14)C-δ-氨基乙酰丙酸掺入叶绿素a和b中。如果匀浆是由已曝光两小时半的黄化子叶制备的,则合成(14)C-叶绿素a。然而,当匀浆由曝光四小时半的子叶制备时,则会产生(14)C-叶绿素a和b。