Koshakji R P, Bush M T, Harbison R D
J Environ Sci Health C. 1979;13(4):315-34.
Pregnant mice were treated with a single oral dose of [carboxy-14C]2,4,5-T (100 mg/kg; 1.22 mu Ci/mg) on day 12 of gestation and sacrificed after 0.25, 0.5, 2 and 24 hours. Maternal blood, embryos, placentas and yolk sacs were analyzed by solvent extraction, TLC, and countercurrent distribution. Expressed as percentage of the administered dose/g tissue, the unchanged 2,4,5-T found in maternal blood, placentas, yolk sacs, and embryos was 3, 0.5, 0.5, and 0.2%, respectively, after 0.25 hours, and 4, 2, 2, and 0.5%, respectively, after 24 hours. No major metabolites of 2,4,5-T were detected. Urine and feces were also collected and analyzed. Radioactivity was largely eliminated in the urine, 69-78% of the administered dose in 7 days. Feces contained 5-9% of the dose. In the urine unchanged 2,4,5-T accounted for 35-44% of the dose, and 22-23% as very polar material. Unchanged 2,4,5-T in the feces was 3-5% and 1-2% as polar material. 2,4,5-T administered to pregnant mice is largely distributed and eliminated as 2,4,5-T and very polar material.
妊娠第12天的孕鼠经口单次给予[羧基 - ¹⁴C]2,4,5 - T（100毫克/千克；1.22微居里/毫克），并于0.25、0.5、2和24小时后处死。通过溶剂萃取、薄层层析和逆流分配法对母血、胚胎、胎盘和卵黄囊进行分析。以给药剂量/克组织的百分比表示，在0.25小时后，母血、胎盘、卵黄囊和胚胎中未变化的2,4,5 - T分别为3%、0.5%、0.5%和0.2%，24小时后分别为4%、2%、2%和0.5%。未检测到2,4,5 - T的主要代谢物。同时收集并分析尿液和粪便。放射性物质主要通过尿液排出，7天内排出给药剂量的69 - 78%。粪便中含有给药剂量的5 - 9%。尿液中未变化的2,4,5 - T占给药剂量的35 - 44%，22 - 23%为极性很强的物质。粪便中未变化的2,4,5 - T为3 - 5%，极性物质为1 - 2%。给予孕鼠的2,4,5 - T主要以2,4,5 - T和极性很强的物质形式分布和排出。
