Romano T J, Ponzio N M, Thorbecke G J
J Immunol. 1976 Jun;116(6):1618-23.
Graft versus host (GVH) reactivity of parental lymph node (LN) cells was assayed by measurements of 3H-thymidine incorporation in vivo. Mitomycin (Mit.) treatment of parental cells abolished their proliferative activity but the combination of such Mit.-treated parental cells with F1 LN cells resulted in much higher proliferation than either one population alone. This recruitment into proliferation of F1 cells was prominent on days 3 and 4 after cell injection and amounted to 35 to 51% of the total activity seen after injection of untreated parental cells alone. The F1 cell sensitive to recruitment was resistant to anti-Thy 1.2 treatment, was not removed by carbonyl iron-magnet separation; and was not present in thymus. The parental cell inducing recruitment was, however, sensitive to anti-Thy 1.2. When spleen cells from hapten immune F1 donors were injected together with Mit.-treated parental LN cells and boosted with hapten on another carrier, a typical "allogeneic effect" was observed in the anti-hapten immune response. It was concluded that Mit.-treated parental T cells exerted a mitogenic effect on F1 B cells resulting in extensive recruitment similar to that seen in murine mixed lymphocyte reactions.
通过体内测量³H-胸腺嘧啶核苷掺入来检测亲代淋巴结（LN）细胞的移植物抗宿主（GVH）反应性。用丝裂霉素（Mit.）处理亲代细胞可消除其增殖活性，但将经Mit.处理的亲代细胞与F1 LN细胞组合后，其增殖程度比单独的任一群体都要高得多。这种F1细胞增殖的募集在细胞注射后第3天和第4天最为显著，相当于单独注射未处理亲代细胞后所见总活性的35%至51%。对募集敏感的F1细胞对抗Thy 1.2处理有抗性，不能通过羰基铁-磁铁分离去除；且不存在于胸腺中。然而，诱导募集的亲代细胞对抗Thy 1.2敏感。当将来自半抗原免疫F1供体的脾细胞与经Mit.处理的亲代LN细胞一起注射，并在另一种载体上用半抗原加强免疫时，在抗半抗原免疫反应中观察到典型的“同种异体效应”。得出的结论是，经Mit.处理的亲代T细胞对F1 B细胞发挥促有丝分裂作用，导致广泛的募集，类似于在小鼠混合淋巴细胞反应中所见。
