通过碱性处理将ADP-核糖转化为5'-AMP及其在微摩尔范围内对单（ADP-核糖）和多（ADP-核糖）残基进行光学定量的应用。

Conversion of ADP-ribose to 5'-AMP by alkaline treatment and its use for an optical quantitation of mono and poly(ADP-ribose) residues in the micromolar range.

作者信息

Goebel M, Stone P R, Lengyel H, Hilz H

出版信息

Hoppe Seylers Z Physiol Chem. 1977 Jan;358(1):13-21. doi: 10.1515/bchm2.1977.358.1.13.

DOI:10.1515/bchm2.1977.358.1.13

Abstract

ADP-Ribose is nearly quantitatively split to 5'-AMP by treatment with alkali at elevated temperatures. This unique behaviour, which is not shown by ADP and other adenine derivatives, was used as the basis of an optical test for the selective determination of ADPR in the presence of other adenine compounds including RNA. Poly(ADPR) could also be quantified when the polymer was degraded by poly(ADPR) glycohydrolase prior to alkaline treatment. When combined with the determination of the terminal AMP residues released by phosphodiesterase I treatment, the chain length of the polymer could be calculated. Application of the method to the quantitation of protein-bound mono(ADPR) residues in Ehrlich ascites tumor cells under different growth conditions is described.

摘要

通过在高温下用碱处理，ADP - 核糖几乎能定量地裂解为5'-AMP。这种独特的行为，ADP和其他腺嘌呤衍生物并不具备，被用作在包括RNA在内的其他腺嘌呤化合物存在下选择性测定ADPR的光学测试基础。当聚（ADPR）在碱性处理前被聚（ADPR）糖苷水解酶降解时，也可以对其进行定量。当与磷酸二酯酶I处理释放的末端AMP残基的测定相结合时，可以计算聚合物的链长。本文描述了该方法在不同生长条件下对艾氏腹水瘤细胞中与蛋白质结合的单（ADPR）残基进行定量的应用。