Rado T A, Cochrane V W
J Bacteriol. 1971 May;106(2):301-4. doi: 10.1128/jb.106.2.301-304.1971.
Extracts prepared from macroconidia of Fusarium solani f. sp. phaseoli are capable, under defined conditions, of incorporating phenylalanine into polypeptide with exogenous polyuridylic acid as messenger. Extracts from ungerminated and germinated spores have approximately the same activity. With endogenous template, leucine incorporation occurs, but in this reaction extracts from germinated spores have about 10 times more activity than do those from ungerminated spores. It is suggested that the low rate in ungerminated spores is attributable to a relative deficiency in the number of ribosomes which are organized into polysomes.
在特定条件下，从菜豆尖镰孢菌大分生孢子制备的提取物能够以外源聚尿苷酸作为信使，将苯丙氨酸掺入多肽中。未萌发和已萌发孢子的提取物具有大致相同的活性。对于内源性模板，会发生亮氨酸掺入，但在该反应中，已萌发孢子的提取物活性比未萌发孢子的提取物高约10倍。有人认为，未萌发孢子中掺入率低是由于组装成多核糖体的核糖体数量相对不足所致。
