Hartline R A, Gunsalus I C
J Bacteriol. 1971 May;106(2):468-78. doi: 10.1128/jb.106.2.468-478.1971.
The ability of bornane and substituted bornanes to induce the early enzymes for d(+)-camphor degradation and control of these enzymes by catabolite repression were studied in a strain of a Pseudomonas putida. Bornane and 20 substituted bornane compounds showed induction. Of these 21 compounds, bornane and 8 of the substituted bornanes provided induction without supporting growth. Oxygen, but not nitrogen, enhanced the inductive potency of the unsubstituted bornane ring. All bornanedione isomers caused induction, and those with substituents on each of the three consecutive carbon atoms, including the methyl group at the bridgehead carbon, showed induction without supporting growth. Although it was not possible to obtain experimental data for a case of absolute gratuitous induction by compounds not supporting growth, indirect evidence in support of gratuitous induction is presented. It is proposed that the ability of P. putida to tolerate the unusually high degree of possible gratuitous induction observed for camphor catabolism may be related to the infrequent occurrence of bicyclic ring structures in nature. Survival of an organism with a broad specificity for gratuitous induction is discussed. Glucose and succinate, but not glutamate, produced catabolite repression of the early camphor-degrading enzymes. Pathway enzymes differ in their degree of sensitivity to succinate-provoked catabolite repression. The ability of a compound to produce catabolite repression is not, however, directly related to the duration of the lag period (diauxic lag) between growth on camphor and growth on the repressing compound.
在一株恶臭假单胞菌中，研究了莰烷及其取代衍生物诱导d(+)-樟脑降解早期酶的能力以及这些酶受分解代谢物阻遏调控的情况。莰烷和20种取代莰烷化合物表现出诱导作用。在这21种化合物中，莰烷和8种取代莰烷能诱导酶产生，但不支持细菌生长。氧气而非氮气增强了未取代莰烷环的诱导效力。所有莰二酮异构体都能引起诱导，那些在三个连续碳原子上都有取代基（包括桥头碳上的甲基）的异构体，在不支持生长的情况下也能诱导酶产生。尽管对于不支持生长的化合物进行绝对的安慰诱导情况无法获得实验数据，但文中提供了支持安慰诱导的间接证据。有人提出，恶臭假单胞菌耐受在樟脑分解代谢中观察到的异常高程度的可能安慰诱导的能力，可能与自然界中双环结构的罕见出现有关。讨论了具有广泛安慰诱导特异性的生物体的生存情况。葡萄糖和琥珀酸而非谷氨酸，对早期樟脑降解酶产生分解代谢物阻遏作用。途径中的酶对琥珀酸引发的分解代谢物阻遏的敏感程度不同。然而，一种化合物产生分解代谢物阻遏的能力与在樟脑上生长和在阻遏化合物上生长之间的延滞期（双相延滞）持续时间并无直接关系。
