Stroun M, Anker P, Gahan P, Rossier A, Greppin H
J Bacteriol. 1971 May;106(2):634-9. doi: 10.1128/jb.106.2.634-639.1971.
Purified Agrobacterium tumefaciens deoxyribonucleic acid (DNA) does not produce crown gall tumors in growing plants, conditioned by wounding, as the living bacteria do. Purified bacterial DNA migrates in the plant and replicates, but it is not transcribed in our experimental conditions. On the contrary, when DNA is released naturally from bacteria into plant cells, a bacterial ribonucleic acid (RNA) can be found in these cells. There seems to be a direct relation between the appearance of A. tumefaciens RNA in the plant cells and the induction of the tumor.
纯化的根癌土壤杆菌脱氧核糖核酸(DNA)不会像活细菌那样,在受创伤影响的生长植物中引发冠瘿瘤。纯化的细菌DNA在植物中迁移并复制,但在我们的实验条件下它不会被转录。相反,当DNA从细菌自然释放到植物细胞中时,在这些细胞中可以发现一种细菌核糖核酸(RNA)。植物细胞中根癌土壤杆菌RNA的出现与肿瘤的诱导之间似乎存在直接关系。