通过间断对流免疫电泳增强血清性肝炎患者中澳大利亚抗原的检测
Enhanced detection of Australia antigen in serum hepatitis patients by discontinuous counter-immunoelectrophoresis.
作者信息
Wallis C, Melnick J L
出版信息
Appl Microbiol. 1971 May;21(5):867-9. doi: 10.1128/am.21.5.867-869.1971.
Abstract
The use of discontinuous counter-immunoelectrophoresis enhanced the reaction between Au/SH antigen and its antibody in agarose. The ionic strength of the Veronal buffer used in the agarose was 0.015 mu, whereas 0.075 mu Veronal (both pH 8.6) was used for anode and cathode buffers. Electroendosmosis is increased under such conditions. Au/SH antigen and antibody reacted to give sharp lines within 30 to 45 min as compared with conventional counter-immunoelectrophoresis which required 1 to 3 hr or longer.
摘要
使用间断性对流免疫电泳增强了金/巯基抗原与其抗体在琼脂糖中的反应。琼脂糖中使用的巴比妥缓冲液的离子强度为0.015μ，而阳极和阴极缓冲液使用的是0.075μ巴比妥缓冲液（均为pH 8.6）。在这种条件下电渗作用增强。与需要1至3小时或更长时间的传统对流免疫电泳相比，金/巯基抗原和抗体在30至45分钟内反应产生清晰的沉淀线。
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本文引用的文献
1
[Immunoelectrophoretic detection of the "hepatitis associated antigen" (Au-SH-antigen)].
Klin Wochenschr. 1970 Jan 1;48(1):58-9. doi: 10.1007/BF01486135.
2
Serum hepatitis antigen (SH): rapid detection by high voltage immunoelectroosmophoresis.
Science. 1970 Aug 7;169(3945):593-5. doi: 10.1126/science.169.3945.593.
3
Rapid detection of Australia antigen by counterimmunoelectrophoresis.
J Immunol. 1970 Apr;104(4):1031-4.
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