流感病毒在不溶性聚电解质上的浓缩与纯化。
Concentration and purification of influenza virus on insoluble polyelectrolytes.
作者信息
Wallis C, Homma A, Melnick J L
出版信息
Appl Microbiol. 1972 Apr;23(4):740-4. doi: 10.1128/am.23.4.740-744.1972.
Abstract
A method for rapid concentration and purification of influenza virus by adsorption on and elution from an insoluble polyelectrolyte is described. To accomplish this task, influenza virus had to be rendered stable at pH 4 to 5, since viruses adsorb to the polyelectrolyte more efficiently at this pH range. A precipitate which forms in influenza harvests under acid conditions in the cold can be removed by ammonium sulfate at a concentration which traps the precipitate but not the virus. Thus, ammonium sulfate-treated influenza virus in allantoic fluid could be readily concentrated on the polyelectrolyte. Elution yielded a virus concentrate essentially free of nonviral proteins.
摘要
描述了一种通过吸附在不溶性聚电解质上并从其上洗脱来快速浓缩和纯化流感病毒的方法。为完成此任务，流感病毒必须在pH 4至5时保持稳定，因为病毒在此pH范围内更有效地吸附到聚电解质上。在寒冷条件下酸性流感收获物中形成的沉淀物可以用硫酸铵去除，该浓度的硫酸铵能捕获沉淀物但不会捕获病毒。因此，尿囊液中经硫酸铵处理的流感病毒可以很容易地浓缩在聚电解质上。洗脱得到的病毒浓缩物基本上不含非病毒蛋白。
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