氯化钠和聚乙二醇（PEG 6000）对非代谢性肾切片含水量的控制

Cortical slices from kidneys of adult rats were equilibrated for 24 and 48 hr in the refrigerator in solutions containing 5 mM-K, 2.5 mM-Ca and Mg, M/15 phosphate buffer (pH 7.4), 5 mM cyanide and iodoacetate with 5, 6, 7 and 8% polyethylene glycol (PEG 6000) and 77, 154, 308, 462 and 770 mM-NaCl.2. There were no consistent differences in water content between slices after 24 and after 48 hr.3. Slices contained more water when either the concentration of NaCl or that of PEG was reduced. The increase in water content induced by lowering the concentration of either was prevented by a higher concentration of the other.4. Water content was held constant at 3.25 kg.kg(-1) dry PEG-free tissue despite a fall in [NaCl] from 770 to 77 mM (more than 1200 m-osmole.kg(-1)) by increasing PEG from 5.1 to 7.7% corresponding to an increase of the order of 15 m-osmole.kg(-1).5. It is suggested that PEG 6000 was so much more effective than NaCl, osmole for osmole, because PEG 6000 acted as a non-penetrating solute, whereas NaCl penetrated the slices and reduced the ;Donnan excess' component of the intracellular colloid osmotic pressure so that this could be balanced by a smaller concentration of PEG.

将成年大鼠肾脏的皮质切片置于含有5 mM - K、2.5 mM - Ca和Mg、M/15磷酸盐缓冲液（pH 7.4）、5 mM氰化物和碘乙酸盐，以及5%、6%、7%和8%聚乙二醇（PEG 6000）和77 mM、154 mM、308 mM、462 mM和770 mM - NaCl的溶液中，在冰箱中平衡24小时和48小时。
24小时和48小时后切片的含水量没有一致的差异。
当NaCl浓度或PEG浓度降低时，切片含有更多的水。降低其中任何一种的浓度所引起的含水量增加可被另一种较高的浓度所阻止。
通过将PEG从5.1%增加到7.7%（相当于增加约15 m - osmole.kg⁻¹），尽管[NaCl]从770 mM降至77 mM（超过1200 m - osmole.kg⁻¹），无PEG的干燥组织的含水量仍保持在3.25 kg.kg⁻¹不变。
有人提出，对于每摩尔渗透压浓度而言，PEG 6000比NaCl有效得多，因为PEG 6000作为一种非渗透性溶质起作用，而NaCl可穿透切片并降低细胞内胶体渗透压的“唐南过量”成分，因此可以用较低浓度的PEG来平衡它。

Control of water content of non-metabolizing kidney slices by sodium chloride and polyethylene glycol (PEG 6000).