Furuya E
Clin Chem. 1977 Jun;23(6):982-5.
Previous papers dealt with the discovery that hydrolysis of 17-hydroxycorticosteroid glucuronides in urine with beta-glucuronidase preparations from bovine liver is increased by adding sodium sulfate to the incubation medium. Here, we conclude that sodium sulfate not only increases the activity of bovine liver beta-glucuronidase on 17-hydroxycorticosteroid glucuronides and p-nitrophenyl glucuronides, but also removes the inhibitory activity of substances of high molecular weight in urine and, moreover, inhibits urine putrefaction during the hydrolysis. The net effect is an increased yield of urinary 17-hydroxycorticosteroids. In the incubation with sodium sulfate (Na2SO4, final concentration 80 g/liter, 500 Fishman units of beta-glucuronidase per milliliter of urine, pH 5.0, 48 degrees C, 18 h) the analytical recovery of 17-hydroxycorticosteroid glucuronides added to 12 urine samples proved to be 98 +/- 1.8% (95-100).
以往的论文探讨了一项发现，即在尿液中用来自牛肝的β-葡萄糖醛酸酶制剂水解17-羟基皮质类固醇葡萄糖醛酸苷时，向孵育介质中添加硫酸钠可提高水解效率。在此，我们得出结论，硫酸钠不仅能提高牛肝β-葡萄糖醛酸酶对17-羟基皮质类固醇葡萄糖醛酸苷和对硝基苯基葡萄糖醛酸苷的活性，还能消除尿液中高分子量物质的抑制活性，此外，在水解过程中抑制尿液腐败。最终结果是尿中17-羟基皮质类固醇的产量增加。在含有硫酸钠（Na2SO4，终浓度80 g/升，每毫升尿液500费什曼单位的β-葡萄糖醛酸酶，pH 5.0，48℃，18小时）进行孵育时，向12份尿液样本中添加的17-羟基皮质类固醇葡萄糖醛酸苷的分析回收率为98±1.8%（95 - 100）。
