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一种使用商业奶粉人工饲养剖腹产出生的幼兔的简化方法(作者译)

[A simplified method using commercial milk powder for hand-rearing of the Caesarean-derived infant rabbits (author's transl)].

作者信息

Murata Y, Tada M, Sugimoto S, Sato M, Katsumata Y

出版信息

Jikken Dobutsu. 1977 Jan;26(1):23-8. doi: 10.1538/expanim1957.26.1_23.

Abstract

An efficient and simplified method for hand-rearing of Caesarean-derived infant rabbits under gnotobiotic condition was devised. The Caesarean-derived infant Dutch or Japanese-White rabbits and their hybrids (F1; Japanese-White female x Dutch male) were reared in sterilized vinyl-isolators by hand-feeding with two kinds of milk diets, A and B, consisted mainly ofa commercial milk powder for dogs and cats (Esbilac) supplemented with several minor components (Table 2) and administered intragastrically once a day through a Nelaton's catheter. Bacteriological examinations revealed that feces and urine were sterile for the first three days. On the third day, Escherichia coli, Staphylococcus epidermidis, Streptococcus faecalis, Bacillus subtilis, Enterococcus, and Bacteroides sp. were given with milk diet and the infant rabbits were reared until 10 to 12 weeks of age. The weaning rate at 5 weeks of age and the raising rate at 3 months of age were approximately 78% and 77%, respectively (Table 3), indicating that feeding once-a-day with a milk diet mainly composed of Esbilac is suitable for the hand-rearing of infant rabbits. There was, however, no significant difference milk diet A and B on the weaning rate.

摘要

设计了一种在无菌条件下人工饲养剖腹产出生的幼兔的高效简化方法。剖腹产出生的荷兰幼兔或日本白兔及其杂种(F1;日本白兔母兔×荷兰公兔)在无菌乙烯隔离器中饲养,通过胃管每天一次人工喂以两种乳类日粮A和B,这两种日粮主要由一种猫狗用商业奶粉(爱思贝丽)补充几种微量成分组成(表2)。细菌学检查显示,最初三天粪便和尿液无菌。在第三天,将大肠杆菌、表皮葡萄球菌、粪肠球菌、枯草芽孢杆菌、肠球菌和拟杆菌属与乳类日粮一起投喂,幼兔饲养至10至12周龄。5周龄时的断奶率和3月龄时的成活率分别约为78%和77%(表3),这表明每天一次用主要由爱思贝丽组成的乳类日粮喂养适合幼兔的人工饲养。然而,日粮A和B在断奶率上没有显著差异。

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