高草酸尿症患者和对照受试者的肾脏和肝脏组织匀浆对1-¹⁴C乙醛酸、1-¹⁴C乙醇酸、1-¹⁴C甘氨酸和2-¹⁴C甘氨酸的代谢情况。
Metabolism of 1-14C glyoxylate, 1-14C glycollate, 1-14C glycine and 2-14C glycine by homogenates of kidney and liver tissue from hyperoxaluric and control subjects.
作者信息
Dean B M, Watts R W, Westwick W J
出版信息
Biochem J. 1967 Nov;105(2):701-7. doi: 10.1042/bj1050701.
Abstract
- The metabolism of [1-(14)C]glyoxylate to carbon dioxide, glycine, oxalate, serine, formate and glycollate was investigated in hyperoxaluric and control subjects' kidney and liver tissue in vitro. 2. Only glycine and carbon dioxide became significantly labelled with (14)C, and this was less in the hyperoxaluric patients' kidney tissue than in the control tissue. 3. Liver did not show this difference. 4. The metabolism of [1-(14)C]glycollate was also studied in the liver tissue; glyoxylate formation was demonstrated and the formation of (14)CO(2) from this substrate was likewise unimpaired in the hyperoxaluric patients' liver tissue in these experiments. 5. Glycine was not metabolized by human kidney, liver or blood cells under the conditions used. 6. These observations show that glyoxylate metabolism by the kidney is impaired in primary hyperoxaluria.
摘要
- 在高草酸尿症患者和对照受试者的肾脏及肝脏组织中，对[1-(14)C]乙醛酸向二氧化碳、甘氨酸、草酸盐、丝氨酸、甲酸盐和乙醇酸盐的代谢进行了体外研究。2. 只有甘氨酸和二氧化碳被显著标记上(14)C，且高草酸尿症患者肾脏组织中的标记程度低于对照组织。3. 肝脏未显示出这种差异。4. 还在肝脏组织中研究了[1-(14)C]乙醇酸盐的代谢；证实了乙醛酸的形成，并且在这些实验中，高草酸尿症患者肝脏组织中由该底物形成(14)CO(2)的过程同样未受损害。5. 在所用条件下，人肾、肝或血细胞均未代谢甘氨酸。6. 这些观察结果表明，原发性高草酸尿症患者肾脏的乙醛酸代谢受损。
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