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阿斯塔西娅中的氧中毒。

Oxygen toxicity in Astasia.

作者信息

Bégin-Heick N, Blum J J

出版信息

Biochem J. 1967 Nov;105(2):813-9. doi: 10.1042/bj1050813.

Abstract
  1. Exposure of Astasia longa to oxygen+carbon dioxide (95:5) at atmospheric pressure leads to an inhibition of growth rate and of respiration. Growth resumes at the normal rate as soon as the oxygenation is discontinued, but respiration recovers more slowly. 2. Mitochondria prepared from cells exposed to oxygen+carbon dioxide (95:5) during growth have considerably decreased activities of succinate-cytochrome c oxidoreductase, NADH-cytochrome c oxidoreductase, succinate dehydrogenase and succinate oxidase activities as compared with mitochondria obtained from cells exposed to air+carbon dioxide (95:5). Cytochrome oxidase activity is not appreciably inhibited by exposure of the cells to 95% oxygen. 3. The mitochondrial fraction of Astasia contains rhodoquinone. The rhodoquinone concentration increases in cells exposed to 95% oxygen. The content of ergosterol-containing compounds also increases in the mitochondria of cells exposed to 95% oxygen. There is little change in the ubiquinone content of the mitochondrial fraction. The ubiquinone of Astasia appears to be ubiquinone-45.
摘要
  1. 在常压下,将长裸藻暴露于氧气+二氧化碳(95:5)中会导致生长速率和呼吸作用受到抑制。一旦停止充氧,生长速率会恢复正常,但呼吸作用恢复得更慢。2. 与从暴露于空气+二氧化碳(95:5)的细胞中获得的线粒体相比,由在生长过程中暴露于氧气+二氧化碳(95:5)的细胞制备的线粒体,其琥珀酸-细胞色素c氧化还原酶、NADH-细胞色素c氧化还原酶、琥珀酸脱氢酶和琥珀酸氧化酶的活性显著降低。细胞暴露于95%氧气中时,细胞色素氧化酶活性并未受到明显抑制。3. 长裸藻的线粒体部分含有玫红醌。在暴露于95%氧气的细胞中,玫红醌浓度增加。在暴露于95%氧气的细胞的线粒体中,含麦角固醇化合物的含量也增加。线粒体部分的泛醌含量变化不大。长裸藻的泛醌似乎是泛醌-45。

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