凝血酶对人洗涤血小板放射性核苷酸的影响。
Effect of thrombin on the radioactive nucleotides of human washed platelets.
作者信息
Ireland D M
出版信息
Biochem J. 1967 Nov;105(2):857-67. doi: 10.1042/bj1050857.
Abstract
Radioactive ATP and ADP were found in platelets after incubation of human platelet-rich plasma with either [8-(14)C]adenosine or [8-(14)C]ADP. Treatment of the labelled and washed platelets with thrombin indicated that, though considerable amounts of ATP and ADP were released to the supernatant, radioactive ATP and ADP remained predominantly in the cellular fraction. Breakdown of radioactive ATP took place to form mainly IMP and hypoxanthine, the latter compound appearing in the supernatant. The results indicate the presence of at least two pools of nucleotide in platelets. Evidence is given that the two pools contain approximately the same amounts of ATP plus ADP, and that the ratio of ATP to ADP in the pool released to the supernatant by the action of thrombin is about 0.7-0.8.
摘要
在富含人血小板的血浆与[8-(14)C]腺苷或[8-(14)C]ADP一起孵育后，在血小板中发现了放射性ATP和ADP。用凝血酶处理标记并洗涤过的血小板表明，尽管大量的ATP和ADP释放到了上清液中，但放射性ATP和ADP仍主要保留在细胞部分。放射性ATP发生分解，主要形成肌苷酸（IMP）和次黄嘌呤，后一种化合物出现在上清液中。结果表明血小板中至少存在两个核苷酸池。有证据表明，这两个池中ATP加ADP的含量大致相同，并且通过凝血酶作用释放到上清液中的池中ATP与ADP的比例约为0.7 - 0.8。
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