不同组织中半胱氨酸和胱氨酸的细胞内比例。
The intracellular ratio of cysteine and cystine in various tissues.
作者信息
Crawhall J C, Segal S
出版信息
Biochem J. 1967 Nov;105(2):891-6. doi: 10.1042/bj1050891.
Abstract
- The cysteine-cystine ratio was measured in rat kidney cortex, diaphragm, jejunum, liver and brain. 2. This ratio was determined by incubating these tissues in buffer containing [(35)S]cystine and then homogenizing the tissue in a buffered solution of N-ethylmaleimide. The products of this reaction were separated by high-voltage electrophoresis and the radioactivity in the cystine and 2-(l-2'-amino-2'-carboxyethylthio)-N-ethylsuccinimide regions was determined. 3. In these tissues cyst(e)ine was mainly present in the reduced form. 4. After incubation of [(35)S]cystine with rat jejunal segments it was found that 36% of the cystine in the medium has been reduced. 5. Anaerobiosis, Na(+)-free media, glucose and high concentrations of cystine and lysine were found not to affect significantly the cysteine-cystine ratio in rat kidney-cortex slices.
摘要
- 测定了大鼠肾皮质、膈肌、空肠、肝脏和脑内的半胱氨酸 - 胱氨酸比率。2. 通过将这些组织在含有[³⁵S]胱氨酸的缓冲液中孵育，然后在N - 乙基马来酰亚胺的缓冲溶液中匀浆来测定该比率。此反应的产物通过高压电泳分离，并测定胱氨酸和2 -（1 - 2'-氨基 - 2'-羧乙基硫代）-N - 乙基琥珀酰亚胺区域的放射性。3. 在这些组织中，半胱氨酸主要以还原形式存在。4. 将[³⁵S]胱氨酸与大鼠空肠段孵育后发现，培养基中36%的胱氨酸已被还原。5. 发现厌氧、无钠培养基、葡萄糖以及高浓度的胱氨酸和赖氨酸对大鼠肾皮质切片中的半胱氨酸 - 胱氨酸比率无显著影响。
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