Wijngaards G, Hemker H C
Haemostasis. 1977;6(2):89-97. doi: 10.1159/000214168.
The one-stage prothrombin time test for tissue thromboplastin activity was evaluated using the correction method. A strong influence of the intrinsic system was seen depending on the clotting time and the type of tubes. This test was unsuitable for quantitative comparison of thromboplastin activity of different preparations. Subsequently a two-stage assay for human thromboplastin is described using purified human factor VII, artifically prepared factor VII reagent, and a phospholipid suspension. The correction method, applicated to this two-stage assay, resulted in a rectilinear dose-response curve when the clotting times of a thromboplastin dilution series were plotted against the reciprocal of the concentrations. Using this test system it could be shown that thromboplastin from porcine tissue can activate human factor VII quite well, while that from bovine tissue cannot.
采用校正法对组织凝血活酶活性的一步法凝血酶原时间试验进行了评估。根据凝血时间和试管类型，观察到内源性系统有强烈影响。该试验不适用于不同制剂凝血活酶活性的定量比较。随后描述了一种使用纯化的人因子VII、人工制备的因子VII试剂和磷脂悬浮液的人凝血活酶两步法测定。当将凝血活酶稀释系列的凝血时间与浓度的倒数作图时，应用于该两步法测定的校正法产生了一条直线剂量-反应曲线。使用该测试系统可以表明，猪组织来源的凝血活酶能很好地激活人因子VII，而牛组织来源的则不能。
