An improved double antibody radioimmunoassay method for the measurement of ragweed antigen E.

Antigen E, the principal antigen of short ragweed pollen, usually has been measured by immunodiffusion or, more recently, by radioimmunoassay. Several technical improvements were made in the radioimmunoassay method which allow the test to be completed in less than a day and which increase sensitivity to 30 picograms per ml of antigen E.