N-methyl-N'-nitro-N-nitrosoguanidine and UV induced mutants of the dinoflagellate Crypthecodinium cohnii.
作者信息
Tuttle R C, Loeblich A R
出版信息
J Protozool. 1977 May;24(2):313-6. doi: 10.1111/j.1550-7408.1977.tb00985.x.
Abstract
Mutant strains were chemically induced by treatment with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) and UV irradiation. UV and NTG mutation rates were obtained that were both consistent with the organism being haploid. Three types of mutants were produced: (a) strains deficient in both beta- and gamma-carotene, the only carotenoids found in the wild type; phenotypes include albinos (translucent, dull white, "snow white") and cream-colored on agar as compared to the yellow-orange color of wild type colonies; (b) strains requiring adenine, guanine or cytosine in addition to the minimal medium for growth; (c) mutants that grow at a rate less than 40% of the wild type in minimal medium.
摘要
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