Goering R V
Mutat Res. 1979 May;60(3):279-89. doi: 10.1016/0027-5107(79)90018-6.
Recombination-deficient (rec) mutants of Staphylococcus aureus strains 152 and Ps29 were sought by initially screening mutagenized cultures for mutants exhibiting increased sensitivity to both ultraviolet (UV) radiation and N-methyl-N'-nitro-N-nitrosoguanidine (NG). Mutants thus isolated were analyzed for recombinational ability by transduction, and further characterized in terms of sensitivity to UV, NG, ability to repair UV-irradiated bacteriophage, and spontaneous and UV-induced DNA degradation. Mutagenesis of strain 152 yielded three isolates, one of which was rec, the second potentially lex, and the third possessing an undetermined repair deficiency. Mutagenesis of strain Ps29 resulted in the isolation of one mutant, which exhibited a rec genotype. In searching for rec mutants of S. aureus, the value of initially screening mutagenized cultures for mutants exhibiting concurrent sensitivity to UV and NG, as opposed to screening for UV sensitivity alone, is discussed.
通过初步筛选诱变培养物，寻找对紫外线（UV）辐射和N-甲基-N'-硝基-N-亚硝基胍（NG）均表现出更高敏感性的突变体，从而获得金黄色葡萄球菌菌株152和Ps29的重组缺陷（rec）突变体。通过转导分析如此分离出的突变体的重组能力，并进一步从对UV、NG的敏感性、修复UV照射噬菌体的能力以及自发和UV诱导的DNA降解方面进行表征。菌株152的诱变产生了三个分离株，其中一个是rec，第二个可能是lex，第三个具有未确定的修复缺陷。菌株Ps29的诱变导致分离出一个突变体，其表现出rec基因型。在寻找金黄色葡萄球菌的rec突变体时，讨论了与仅筛选UV敏感性相反，初步筛选诱变培养物以寻找对UV和NG同时敏感的突变体的价值。
