脑心肌炎病毒复制酶的分离与特性
Isolation and properties of the replicase of encephalomyocarditis virus.
作者信息
Traub A, Duskin B, Rosenberg H, Kalmar E
出版信息
J Virol. 1976 May;18(2):375-82. doi: 10.1128/JVI.18.2.375-382.1976.
Abstract
The RNA-dependent RNA polymerase (replicase) of encephalomyocarditis (EMC) virus was found to be closely associated with the smooth membranes of infected BHK-21 cells. An RNA-dependent EMC replicase was extracted from the membranes with 0.15% sodium dodecyl sulfate (SDS) and 1,1,2-trichlorotri-fluoroethane (Genetron 113) and further purified by high-salt dextran-polyethylene glycol phase separation, sievorptive chromatography, and glycerol gradient sedimentation. The enzyme does not manifest strict specificity toward EMC RNA template. It can use also Qbeta RNA, rRNA of BHK cells, or poly(C). SDS-polyacrylamide gel electrophoresis of purified EMC replicase labeled with radioactive methionine revealed that, of all the stable EMC proteins, the enzyme contains predominantly the 56,000-dalton (E) polypeptide.
摘要
有人发现脑心肌炎（EMC）病毒的RNA依赖性RNA聚合酶（复制酶）与被感染的BHK - 21细胞的光滑膜紧密相关。用0.15%的十二烷基硫酸钠（SDS）和1,1,2 - 三氯三氟乙烷（Genetron 113）从膜中提取RNA依赖性EMC复制酶，并通过高盐葡聚糖 - 聚乙二醇相分离、吸附色谱法和甘油梯度沉降进一步纯化。该酶对EMC RNA模板没有严格的特异性。它也可以使用Qβ RNA、BHK细胞的rRNA或聚（C）。用放射性甲硫氨酸标记的纯化EMC复制酶的SDS - 聚丙烯酰胺凝胶电泳显示，在所有稳定的EMC蛋白中，该酶主要含有56,000道尔顿（E）的多肽。
相似文献
1
Isolation and properties of the replicase of encephalomyocarditis virus.脑心肌炎病毒复制酶的分离与特性
J Virol. 1976 May;18(2):375-82. doi: 10.1128/JVI.18.2.375-382.1976.
2
Isolation and subunit structure of polycytidylate-dependent RNA polymerase of encephalomyocarditis virus.
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3815-9. doi: 10.1073/pnas.69.12.3815.
3
Mechanism of interferon action: inhibition of viral messenger ribonucleic acid translation in L-cell extracts.干扰素作用机制：对L细胞提取物中病毒信使核糖核酸翻译的抑制作用
J Virol. 1972 Dec;10(6):1184-98. doi: 10.1128/JVI.10.6.1184-1198.1972.
4
Synthesis of capsid and noncapsid viral proteins in response to encephalomyocarditis virus ribonucleic acid in animal cell-free systems.在无细胞动物系统中，响应脑心肌炎病毒核糖核酸合成衣壳蛋白和非衣壳病毒蛋白。
J Virol. 1971 Oct;8(4):491-9. doi: 10.1128/JVI.8.4.491-499.1971.
5
Poliovirus polyuridylic acid polymerase and RNA replicase have the same viral polypeptide.脊髓灰质炎病毒聚尿苷酸聚合酶和RNA复制酶具有相同的病毒多肽。
J Virol. 1979 Jan;29(1):352-60. doi: 10.1128/JVI.29.1.352-360.1979.
6
Expression, purification, and properties of recombinant encephalomyocarditis virus RNA-dependent RNA polymerase.重组脑心肌炎病毒RNA依赖性RNA聚合酶的表达、纯化及特性
J Virol. 1991 Jun;65(6):2993-3000. doi: 10.1128/JVI.65.6.2993-3000.1991.
7
Encephalomyocarditis virus RNA polymerase preparations, with and without RNA helicase activity.
J Virol. 1991 May;65(5):2714-7. doi: 10.1128/JVI.65.5.2714-2717.1991.
8
Studies on the replicase of encephalomyocarditis virus.
Adv Exp Med Biol. 1974 Jun;44(1):295-308. doi: 10.1007/978-1-4684-3246-6_19.
9
引用本文的文献
1
2
Isolation and Characterization of an RNA-Dependent RNA Polymerase from Black Beetle Virus-Infected Drosophila melanogaster Cells.从感染黑甲虫病毒的果蝇细胞中分离和鉴定一种依赖RNA的RNA聚合酶
J Virol. 1981 Nov;40(2):379-86. doi: 10.1128/JVI.40.2.379-386.1981.
3
In Vitro Replication of Cowpea Mosaic Virus RNA III. Template Recognition by Cowpea Mosaic Virus RNA Replicase.豇豆花叶病毒RNA III的体外复制。豇豆花叶病毒RNA复制酶对模板的识别。
J Virol. 1979 Jan;29(1):21-33. doi: 10.1128/JVI.29.1.21-33.1979.
4
Picornaviral structure and assembly.小核糖核酸病毒的结构与组装
Microbiol Rev. 1981 Jun;45(2):287-315. doi: 10.1128/mr.45.2.287-315.1981.
5
Evidence for intramolecular self-cleavage of picornaviral replicase precursors.微小核糖核酸病毒复制酶前体分子内自我切割的证据。
J Virol. 1982 Jan;41(1):244-9. doi: 10.1128/JVI.41.1.244-249.1982.
6
Identification of the polyprotein termination site on encephalomyocarditis viral RNA.脑心肌炎病毒RNA上多聚蛋白终止位点的鉴定。
J Virol. 1982 Feb;41(2):726-9. doi: 10.1128/JVI.41.2.726-729.1982.
7
Characterization of a 70S polyuridylic acid polymerase isolated from foot-and-mouth disease virus-infected cells.从口蹄疫病毒感染细胞中分离出的70S多聚尿苷酸聚合酶的特性分析。
J Virol. 1981 Dec;40(3):881-9. doi: 10.1128/JVI.40.3.881-889.1981.
8
Isolation of a foot-and-mouth disease polyuridylic acid polymerase and its inhibition by antibody.口蹄疫多聚尿苷酸聚合酶的分离及其抗体抑制作用
J Virol. 1980 Feb;33(2):774-9. doi: 10.1128/JVI.33.2.774-779.1980.
9
Implication of mitochondria in the replication of Nodamura virus in larvae of the Lepidoptera, Galleria mellonella (L.) and in suckling mice.线粒体在诺达木病毒于鳞翅目昆虫大蜡螟幼虫及乳鼠体内复制中的作用
Arch Virol. 1990;113(3-4):165-76. doi: 10.1007/BF01316670.
10
Expression, purification, and properties of recombinant encephalomyocarditis virus RNA-dependent RNA polymerase.重组脑心肌炎病毒RNA依赖性RNA聚合酶的表达、纯化及特性
J Virol. 1991 Jun;65(6):2993-3000. doi: 10.1128/JVI.65.6.2993-3000.1991.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
STUDIES ON EMC VIRAL RNA SYNTHESIS AND ITS LOCALIZATION IN INFECTED KREBS ASCITES CELLS.埃可病毒在感染的克雷布斯腹水癌细胞中的RNA合成及其定位研究
Virology. 1965 Jul;26:450-65. doi: 10.1016/0042-6822(65)90008-5.
3
AN RNA "REPLICASE" INDUCED BY AND SELECTIVE FOR A VIRAL RNA: ISOLATION AND PROPERTIES.一种由病毒RNA诱导并对其具有选择性的RNA“复制酶”：分离与特性
Proc Natl Acad Sci U S A. 1963 Nov;50(5):905-11. doi: 10.1073/pnas.50.5.905.
4
The biosynthesis of ribonucleic acid following infection with a ribonucleic acid virus.
J Biol Chem. 1963 May;238:1788-92.
5
Induction by an RNA phage of an enzyme catalyzing incorporation of ribonucleotides into ribonucleic acid.一种RNA噬菌体诱导催化核糖核苷酸掺入核糖核酸的酶的产生。
Proc Natl Acad Sci U S A. 1963 Mar 15;49(3):407-14. doi: 10.1073/pnas.49.3.407.
6
Preliminary data on a virus-specific enzyme system responsible for the synthesis of viral RNA.关于负责病毒RNA合成的病毒特异性酶系统的初步数据。
Biochem Biophys Res Commun. 1962 Nov 27;9:388-92. doi: 10.1016/0006-291x(62)90021-9.
7
Patterns of macromolecular synthesis in normal and virus-infected mammalian cells.正常及病毒感染的哺乳动物细胞中大分子合成的模式
Cold Spring Harb Symp Quant Biol. 1962;27:175-98. doi: 10.1101/sqb.1962.027.001.019.
8
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
9
Characterization of the subunits of Q-beta replicase.Q-β复制酶亚基的表征
Nature. 1970 Nov 7;228(5271):527-33. doi: 10.1038/228527a0.
10
Subunit structure of Q-beta replicase.Q-β复制酶的亚基结构。
Nature. 1970 Nov 7;228(5271):525-7. doi: 10.1038/228525a0.
Zotero 插件