脑心肌炎病毒复制酶的分离与特性
Isolation and properties of the replicase of encephalomyocarditis virus.
作者信息
Traub A, Duskin B, Rosenberg H, Kalmar E
出版信息
J Virol. 1976 May;18(2):375-82. doi: 10.1128/JVI.18.2.375-382.1976.
Abstract
The RNA-dependent RNA polymerase (replicase) of encephalomyocarditis (EMC) virus was found to be closely associated with the smooth membranes of infected BHK-21 cells. An RNA-dependent EMC replicase was extracted from the membranes with 0.15% sodium dodecyl sulfate (SDS) and 1,1,2-trichlorotri-fluoroethane (Genetron 113) and further purified by high-salt dextran-polyethylene glycol phase separation, sievorptive chromatography, and glycerol gradient sedimentation. The enzyme does not manifest strict specificity toward EMC RNA template. It can use also Qbeta RNA, rRNA of BHK cells, or poly(C). SDS-polyacrylamide gel electrophoresis of purified EMC replicase labeled with radioactive methionine revealed that, of all the stable EMC proteins, the enzyme contains predominantly the 56,000-dalton (E) polypeptide.
摘要
有人发现脑心肌炎（EMC）病毒的RNA依赖性RNA聚合酶（复制酶）与被感染的BHK - 21细胞的光滑膜紧密相关。用0.15%的十二烷基硫酸钠（SDS）和1,1,2 - 三氯三氟乙烷（Genetron 113）从膜中提取RNA依赖性EMC复制酶，并通过高盐葡聚糖 - 聚乙二醇相分离、吸附色谱法和甘油梯度沉降进一步纯化。该酶对EMC RNA模板没有严格的特异性。它也可以使用Qβ RNA、BHK细胞的rRNA或聚（C）。用放射性甲硫氨酸标记的纯化EMC复制酶的SDS - 聚丙烯酰胺凝胶电泳显示，在所有稳定的EMC蛋白中，该酶主要含有56,000道尔顿（E）的多肽。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
STUDIES ON EMC VIRAL RNA SYNTHESIS AND ITS LOCALIZATION IN INFECTED KREBS ASCITES CELLS.埃可病毒在感染的克雷布斯腹水癌细胞中的RNA合成及其定位研究
Virology. 1965 Jul;26:450-65. doi: 10.1016/0042-6822(65)90008-5.
3
AN RNA "REPLICASE" INDUCED BY AND SELECTIVE FOR A VIRAL RNA: ISOLATION AND PROPERTIES.一种由病毒RNA诱导并对其具有选择性的RNA“复制酶”：分离与特性
Proc Natl Acad Sci U S A. 1963 Nov;50(5):905-11. doi: 10.1073/pnas.50.5.905.
4
The biosynthesis of ribonucleic acid following infection with a ribonucleic acid virus.
J Biol Chem. 1963 May;238:1788-92.
5
Induction by an RNA phage of an enzyme catalyzing incorporation of ribonucleotides into ribonucleic acid.一种RNA噬菌体诱导催化核糖核苷酸掺入核糖核酸的酶的产生。
Proc Natl Acad Sci U S A. 1963 Mar 15;49(3):407-14. doi: 10.1073/pnas.49.3.407.
6
Preliminary data on a virus-specific enzyme system responsible for the synthesis of viral RNA.关于负责病毒RNA合成的病毒特异性酶系统的初步数据。
Biochem Biophys Res Commun. 1962 Nov 27;9:388-92. doi: 10.1016/0006-291x(62)90021-9.
7
Patterns of macromolecular synthesis in normal and virus-infected mammalian cells.正常及病毒感染的哺乳动物细胞中大分子合成的模式
Cold Spring Harb Symp Quant Biol. 1962;27:175-98. doi: 10.1101/sqb.1962.027.001.019.
8
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
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