Gosden J R, Mitchell A R, Seuanez H N, Gosden C M
Chromosoma. 1977 Sep 27;63(3):253-71. doi: 10.1007/BF00327453.
Human satellite DNAs I, II and IV were transcribed to yield radioactive complementary RNAs (cRNAs). These cRNAs were hybridised to metaphase chromosomes of man, chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla) and orang utan (Pongo pygmaeus). The results of this in situ hybridisation were analysed quantitatively and compared with accepted chromosome homologies based on Giemsa banding patterns. The cRNA to satellite II (cRNAII) did not hybridise to chimpanzee chromosomes, although its hybridisation to chromosomes of gorilla and orang utan yielded more autoradiograph grains than hybridisation to human chromosomes, and cRNAIV hybridised to many chromosomes of gorilla and chimpanzee but was almost entirely restricted to the Y chromosome in orang utan. Most sites of hybridisation were located on homologous chromosomes in all four species, but there were a number of sites which showed no correspondence between satellite DNA location and chromosome banding patterns, and others where a given chromosomal location hybridised with different cRNAs in each species. These results are in contrast to those found for many transcribed DNA sequences, where the same sequence is usually located at homologous chromosome sites in different species, and appear to cast doubt on many proposed models of satellite DNA function.
人类卫星DNA I、II和IV被转录以产生放射性互补RNA（cRNA）。这些cRNA与人类、黑猩猩（Pan troglodytes）、大猩猩（Gorilla gorilla）和红毛猩猩（Pongo pygmaeus）的中期染色体进行杂交。对这种原位杂交的结果进行了定量分析，并与基于吉姆萨带型的公认染色体同源性进行了比较。卫星II的cRNA（cRNAII）未与黑猩猩染色体杂交，尽管其与大猩猩和红毛猩猩染色体的杂交产生的放射自显影片颗粒比与人类染色体杂交的多，并且cRNAIV与大猩猩和黑猩猩的许多染色体杂交，但在红毛猩猩中几乎完全局限于Y染色体。在所有四个物种中，大多数杂交位点位于同源染色体上，但有许多位点在卫星DNA位置和染色体带型之间没有对应关系，还有一些位点在每个物种中给定的染色体位置与不同的cRNA杂交。这些结果与许多转录DNA序列的结果形成对比，在许多转录DNA序列中，相同的序列通常位于不同物种的同源染色体位点上，并且似乎对许多提出的卫星DNA功能模型提出了质疑。
