多头绒泡菌中肌球蛋白的合成与运输。

Synthesis and transport of myosin in Physarum polycephalum.

作者信息

Schwärzler M, Jockusch B M, Hall L, Braun R

出版信息

Eur J Biochem. 1977 Oct 17;80(1):43-50. doi: 10.1111/j.1432-1033.1977.tb11853.x.

DOI:10.1111/j.1432-1033.1977.tb11853.x

Abstract

Immunological techniques have been used to study the rate of synthesis and intracellular transport of myosin in the slime mould Physarum polycephalum. Quantitative precipitation of myosin in homogenates of Physarum was achieved using an antimyosin antibody produced in rabbit in response to purified Physarum myosin. Dodecylsulphate-gel electrophoresis revealed that about 50% of the precipitated material is myosin. The rates of synthesis of total cellular protein and myosin were measured over the mitotic cycle. Both were found to increase exponentially or linearly between two successive nuclear divisions. Similarly, no difference in the proportion of myosin-synthesising polysomes, assayed by precipitation with antimyosin serum, could be detected between the S phase and G2 phase of the mitotic cycle. Myosin makes up nearly 2% of total plasmodial proteins. Its transport into the nucleus occurs predominantly during the G2 phase.

摘要

免疫技术已被用于研究多头绒泡菌中肌球蛋白的合成速率和细胞内运输。利用兔体内针对纯化的多头绒泡菌肌球蛋白产生的抗肌球蛋白抗体，实现了多头绒泡菌匀浆中肌球蛋白的定量沉淀。十二烷基硫酸钠 - 凝胶电泳显示，沉淀物质中约50%是肌球蛋白。在有丝分裂周期中测量了总细胞蛋白和肌球蛋白的合成速率。发现在两个连续的核分裂之间，两者均呈指数或线性增加。同样，通过抗肌球蛋白血清沉淀测定，在有丝分裂周期的S期和G2期之间，未检测到合成肌球蛋白的多核糖体比例有差异。肌球蛋白占总原质体蛋白的近2%。它向细胞核的运输主要发生在G2期。