Trenkner E, Sidman R L
J Cell Biol. 1977 Dec;75(3):915-40. doi: 10.1083/jcb.75.3.915.
A microwell culture system was developed for analysis of cell movements and interactions during nervous system histogenesis. Cells from trypsinized 7-day-old mouse cerebellum reaggregated within hours into clusters which later developed interconnections consisting of either sheets of migrating cells and cell processes or cables of fiber bundles with cells migrating along their surfaces. Granule cells in several stages of differentiation, basket and/or stellate neurons, some larger neurons, and two types of neuroglial cells were identified in reproducible, nonrandom patterns by scanning and transmission electron microscopy. Axonal and dendritic processes, both with growth cones, and numerous synapses were generated in vitro.
开发了一种微孔培养系统，用于分析神经系统组织发生过程中的细胞运动和相互作用。来自7日龄小鼠小脑经胰蛋白酶消化的细胞在数小时内重新聚集形成簇，这些簇随后发展出由迁移细胞片和细胞突起组成的互连结构，或由纤维束组成的索状结构，细胞沿其表面迁移。通过扫描电子显微镜和透射电子显微镜，以可重复的、非随机的模式鉴定出处于几个分化阶段的颗粒细胞、篮状和/或星状神经元、一些较大的神经元以及两种神经胶质细胞。体外产生了带有生长锥的轴突和树突突起以及大量突触。
