Dortaj Hengameh, Yadegari Maryam, Hosseini Sharif Abad Mohammad, Abbasi Sarcheshmeh Abolghasem, Anvari Morteza
Department of Anatomy and Cell Biology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
Basic Clin Neurosci. 2018 Jan-Feb;9(1):27-34. doi: 10.29252/nirp.bcn.9.1.27.
Acrylamide (ACR) consumption is increasing all over the world. There are some evidence on the literature about its neurotoxic effect on mature animals, but the effects of ACR on postnatal development have been less studied. The purpose of this study was to evaluate the effects of ACR on development of cortical layer, white matter, and number of Purkinje cells of the cerebellum in rat newborns.
This study was carried out on 20 female Wistar rats (average weight: 180 g, aged: two months). The rats were divided into four groups. Pregnant rats were orally fed with ACR 10 mg/kg and vitamin C 200 mg/kg. In this study, 6 infants of each group (weighting 32-35 g) were randomly selected at day 21 after birth and placed under deep anesthesia and transcardial perfusion. Their cerebellums were fixed and histopathological changes were evaluated with Hematoxylin and Eosin (H&E) staining and cresyl violet method. The volume of cerebellar cortical layers and number of Purkinje cells were investigated by Cavalieri's principle and physical dissector methods. The obtained data were analyzed by 1-way ANOVA and LSD test using SPSS. P<0.05 considered as statistically significant.
The results showed that newborns of ACR-treated female rats have decreased cerebellar weight (P≤0.05) and lower than average number of Purkinje cells (P≤0.001). ACR also decreased the volume of granular and molecular layer and increased the volume of white matter. While the results showed decreased in white matter volume in vitamin C group (P≤0.001).
ACR induces structural changes in the development of the cerebellar cortical layers in rat newborns, but these changes may be prevented by vitamin C as an antioxidant.
全球范围内丙烯酰胺（ACR）的摄入量正在增加。文献中有一些关于其对成年动物神经毒性作用的证据，但ACR对产后发育的影响研究较少。本研究的目的是评估ACR对新生大鼠小脑皮质层、白质发育及浦肯野细胞数量的影响。
本研究对20只雌性Wistar大鼠（平均体重：180 g，年龄：两个月）进行。将大鼠分为四组。给怀孕大鼠口服10 mg/kg的ACR和200 mg/kg的维生素C。在本研究中，每组6只幼崽（体重32 - 35 g）在出生后第21天被随机选取，置于深度麻醉下进行经心灌注。将它们的小脑固定，并用苏木精和伊红（H&E）染色及甲酚紫法评估组织病理学变化。通过卡瓦列里原理和物理分割法研究小脑皮质层的体积和浦肯野细胞的数量。使用SPSS软件通过单因素方差分析和LSD检验对所得数据进行分析。P<0.05被认为具有统计学意义。
结果表明，经ACR处理的雌性大鼠的新生幼崽小脑重量降低（P≤0.05），浦肯野细胞数量低于平均水平（P≤0.001）。ACR还减少了颗粒层和分子层的体积，增加了白质的体积。而结果显示维生素C组白质体积减少（P≤0.001）。
ACR可诱导新生大鼠小脑皮质层发育的结构变化，但作为抗氧化剂的维生素C可能会预防这些变化。
